Am J Physiol Cell Physiol AJP: Renal Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Am J Physiol Cell Physiol (September 11, 2002). doi:10.1152/ajpcell.00247.2002
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
284/1/C119    most recent
00247.2002v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ragolia, L.
Right arrow Articles by Maesaka, J. K
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ragolia, L.
Right arrow Articles by Maesaka, J. K

Articles in PresS, published online ahead of print September 11, 2002
Am J Physiol Cell Physiol, 10.1152/ajpcell.00247.2002
Submitted on May 29, 2002
Accepted on December 31, 1969

Elevated Prostaglandin D2 Synthase Activity Contributes to PMA-induced Apoptosis in LLC-PK1 Cells Concomitant with Down-regulation of PI3-K

Louis Ragolia1*, Thomas Palaia2, Enesa Paric2, and John K Maesaka1

1 Cell Biology, Winthrop-University Hospital, Mineola, NY, USA; Medicine, Stony Brook University, Stony Brook, NY, USA
2 Cell Biology, Winthrop-University Hospital, Mineola, NY, USA

* To whom correspondence should be addressed. E-mail: lragolia{at}winthrop.org.

Recently, we demonstrated the induction of apoptosis by the addition of recombinant prostaglandin D2 synthase (L-PGDS) to the culture medium of LLC-PK1 cells. Since protein kinase C (PKC) has been shown to be involved in the apoptotic process of various cell types, we examined the potential role of L-PGDS in PMA-induced apoptosis. We report here the enzymatic activation and phosphorylation of L-PGDS, in response to phorbol ester in cell culture, and the direct phosphorylation of recombinant L-PGDS by PKC in vitro. Treatment of cells with PMA or L-PGDS, decreased phosphatidylinositol 3-kinase (PI3-K) activity and concomitantly inhibited protein kinase B (PKB/Akt) phosphorylation, leading to the hypophosphorylation and activation of Bad. In addition, hypophosphorylation of retinoblastoma protein (pRb) was also observed in response to L-PGDS-induced apoptosis. Cellular depletion of L-PGDS levels, using an antisense RNA strategy, prevented PI3-K inactivation by phorbol ester and inhibited caspase3 activation and apoptosis. We conclude that phorbol ester-induced apoptosis is mediated by L-PGDS phosphorylation and activation by PKC, and is accompanied by an inhibition of the PI3-K/PKB anti-apoptotic signaling pathways.




This article has been cited by other articles:


Home page
 BrainHome page
H. E. Killer, G. P. Jaggi, J. Flammer, N. R. Miller, A. R. Huber, and A. Mironov
Cerebrospinal fluid dynamics between the intracranial and the subarachnoid space of the optic nerve. Is it always bidirectional?
Brain, February 1, 2007; 130(2): 514 - 520.
[Abstract] [Full Text] [PDF]

Home page
 BrainHome page
H. E. Killer, G. P. Jaggi, J. Flammer, N. R. Miller, and A. R. Huber
The optic nerve: a new window into cerebrospinal fluid composition?
Brain, April 1, 2006; 129(4): 1027 - 1030.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
J. Zhang, C. P. Baines, C. Zong, E. M. Cardwell, G. Wang, T. M. Vondriska, and P. Ping
Functional proteomic analysis of a three-tier PKC{varepsilon}-Akt-eNOS signaling module in cardiac protection
Am J Physiol Heart Circ Physiol, February 1, 2005; 288(2): H954 - H961.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
L. Ragolia, T. Palaia, T. B. Koutrouby, and J. K. Maesaka
Inhibition of cell cycle progression and migration of vascular smooth muscle cells by prostaglandin D2 synthase: resistance in diabetic Goto-Kakizaki rats
Am J Physiol Cell Physiol, November 1, 2004; 287(5): C1273 - C1281.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Visit Other APS Journals Online
Copyright © 1976 by the American Physiological Society.