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Am J Physiol Cell Physiol (October 3, 2007). doi:10.1152/ajpcell.00227.2007
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Submitted on June 1, 2007
Accepted on September 19, 2007

The proliferation capacity of the renal proximal tubule involves the bulk of differentiated epithelial cells

Alexander Vogetseder1, Nicolas Picard1, Ariana Gaspert2, Michael Walch1, Brigitte Kaissling1, and Michel Le Hir1*

1 Anatomy, University of Zurich, Zurich, Switzerland
2 Pathology, University Hospital Zurich, Zurich, Switzerland

* To whom correspondence should be addressed. E-mail: lehir{at}anatom.uzh.ch.

We investigated the proliferative capacity of renal proximal tubular cells in healthy rats. Previously we observed that tubular cells originate from differentiated cells. We now found 1. by application of bromo-deoxyuridine (BrdU) for 14 days and co-staining for BrdU and the G1-phase marker cyclin D1 that the bulk of cells in the S3 segment of juvenile rats were involved in proliferation; 2. that although the proliferation rate was about 10-fold higher in juvenile compared to adult rats, roughly 40% of S3 cells were in G1 in both groups; 3. that after a strong mitotic stimulus (lead acetate) proliferation was similar in juveniles and adults; 4. that there was a high incidence of cyclin D1-positive cells also in the healthy human kidney; 5. by labelling dividing cells with BrdU for 2 days before applying lead acetate and subsequent co-staining for BrdU and cell cycle markers, that, although a strong mitotic stimulus does not abolish the period of quiescence following division, it shortens it markedly. Thus, the capacity of the proximal tubule to rapidly recruit cells into division relies on a large reserve pool of cells in G1 and on the shortening of the obligatory period of quiescence which follows division.




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