| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1 Orthopaedics and Bioengineering, University of California, La Jolla, CA, USA
2 Surgery, University of California, La Jolla, CA, USA
3 Medicine, University of California, La Jolla, CA, USA
* To whom correspondence should be addressed. E-mail: rlieber{at}ucsd.edu.
Eccentric contractions (ECs), in which a muscle is forced to lengthen while activated, result in muscle injury and, eventually, muscle strengthening and prevention of further injury. Although the mechanical basis of eccentric contraction-induced injury has been studied in detail, muscle's biological response is less well characterized. This study presents the development of a minimally-invasive model of EC injury in the mouse, follows the time course of torque recovery after an injurious bout of ECs, and uses Affymetrix microarrays to compare the gene expression profile 48 hours after ECs to both isometrically stimulated muscles and contralateral muscles. Torque dropped by about 55% immediately after the exercise bout, and recovered to initial levels 7 days later. 36 known genes were upregulated after ECs compared to contralateral and isometrically stimulated muscles, including five muscle specific genes: muscle LIM protein (MLP), Muscle Ankyrin Repeat Proteins (MARP 1 and 2; also known as cardiac ankyrin repeat protein and Arpp/Ankrd2, respectively), Xin, and Myosin Binding Protein H. The time courses of MLP and MARP expression after the injury bout (determined by quantitative real-time polymerase chain reaction) indicate that these genes are rapidly induced, reaching a peak expression level of 6-11 times contralateral values 12-24 hours after the EC bout and returning to baseline within 72 hours. Very little gene induction was seen after either isometric activation or passive stretch, indicating that the MLP and MARP genes may play an important and specific role in the biological response of muscle to EC-induced injury.
This article has been cited by other articles:
|
|
 |
|
  C. M. Stary, B. J. Walsh, A. E. Knapp, D. Brafman, and M. C. Hogan Elevation in heat shock protein 72 mRNA following contractions in isolated single skeletal muscle fibers Am J Physiol Regulatory Integrative Comp Physiol, August 1, 2008; 295(2): R642 - R648. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. J. Mahoney, A. Safdar, G. Parise, S. Melov, M. Fu, L. MacNeil, J. Kaczor, E. T. Payne, and M. A. Tarnopolsky Gene expression profiling in human skeletal muscle during recovery from eccentric exercise Am J Physiol Regulatory Integrative Comp Physiol, June 1, 2008; 294(6): R1901 - R1910. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. R. Stone, A. O'Neill, R. M. Lovering, J. Strong, W. G. Resneck, P. W. Reed, D. M. Toivola, J. A. Ursitti, M. B. Omary, and R. J. Bloch Absence of keratin 19 in mice causes skeletal myopathy with mitochondrial and sarcolemmal reorganization J. Cell Sci., November 15, 2007; 120(22): 3999 - 4008. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. J. Hawke, D. J. Atkinson, S. B. Kanatous, P. F. M. Van der Ven, S. C. Goetsch, and D. J. Garry Xin, an actin binding protein, is expressed within muscle satellite cells and newly regenerated skeletal muscle fibers Am J Physiol Cell Physiol, November 1, 2007; 293(5): C1636 - C1644. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. C. Kostek, Y.-W. Chen, D. J. Cuthbertson, R. Shi, M. J. Fedele, K. A. Esser, and M. J. Rennie Gene expression responses over 24 h to lengthening and shortening contractions in human muscle: major changes in CSRP3, MUSTN1, SIX1, and FBXO32 Physiol Genomics, September 11, 2007; 31(1): 42 - 52. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. M. Crameri, P. Aagaard, K. Qvortrup, H. Langberg, J. Olesen, and M. Kjaer Myofibre damage in human skeletal muscle: effects of electrical stimulation versus voluntary contraction J. Physiol., August 15, 2007; 583(1): 365 - 380. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. L. Warren, M. Summan, X. Gao, R. Chapman, T. Hulderman, and P. P. Simeonova Mechanisms of skeletal muscle injury and repair revealed by gene expression studies in mouse models J. Physiol., July 15, 2007; 582(2): 825 - 841. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. A. Barash, M.-L. Bang, L. Mathew, M. L. Greaser, J. Chen, and R. L. Lieber Structural and regulatory roles of muscle ankyrin repeat protein family in skeletal muscle Am J Physiol Cell Physiol, July 1, 2007; 293(1): C218 - C227. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. A. Clark, J. M. Bland, and M. C. Beckerle The Drosophila muscle LIM protein, Mlp84B, cooperates with D-titin to maintain muscle structural integrity J. Cell Sci., June 15, 2007; 120(12): 2066 - 2077. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. A. Smith, F. Stauber, C. Waters, S. E. Alway, and W. T. Stauber Transforming growth factor-beta following skeletal muscle strain injury in rats J Appl Physiol, February 1, 2007; 102(2): 755 - 761. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. M. Lehti, M. Silvennoinen, R. Kivela, H. Kainulainen, and J. Komulainen Effects of streptozotocin-induced diabetes and physical training on gene expression of titin-based stretch-sensing complexes in mouse striated muscle Am J Physiol Endocrinol Metab, February 1, 2007; 292(2): E533 - E542. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. G. Tidball and M. Wehling-Henricks Macrophages promote muscle membrane repair and muscle fibre growth and regeneration during modified muscle loading in mice in vivo J. Physiol., January 1, 2007; 578(1): 327 - 336. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. H. Mortensen, L. Frandsen, P. Schjerling, E. Nishimura, and N. Grunnet PGC-1{alpha} and PGC-1beta have both similar and distinct effects on myofiber switching toward an oxidative phenotype Am J Physiol Endocrinol Metab, October 1, 2006; 291(4): E807 - E816. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Raffaello, P. Laveder, C. Romualdi, C. Bean, L. Toniolo, E. Germinario, A. Megighian, D. Danieli-Betto, C. Reggiani, and G. Lanfranchi Denervation in murine fast-twitch muscle: short-term physiological changes and temporal expression profiling Physiol Genomics, March 13, 2006; 25(1): 60 - 74. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. R. Hentzen, M. Lahey, D. Peters, L. Mathew, I. A. Barash, J. Friden, and R. L. Lieber Stress-dependent and -independent expression of the myogenic regulatory factors and the MARP genes after eccentric contractions in rats J. Physiol., January 1, 2006; 570(1): 157 - 167. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Choi, X. Liu, P. Li, T. Akimoto, S. Y. Lee, M. Zhang, and Z. Yan Transcriptional profiling in mouse skeletal muscle following a single bout of voluntary running: evidence of increased cell proliferation J Appl Physiol, December 1, 2005; 99(6): 2406 - 2415. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. A. Barash, L. Mathew, M. Lahey, M. L. Greaser, and R. L. Lieber Muscle LIM protein plays both structural and functional roles in skeletal muscle Am J Physiol Cell Physiol, November 1, 2005; 289(5): C1312 - C1320. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. A. Butterfield and W. Herzog Quantification of muscle fiber strain during in vivo repetitive stretch-shortening cycles J Appl Physiol, August 1, 2005; 99(2): 593 - 602. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Mckoy, Y. Hou, S. Y. Yang, D. Vega Avelaira, H. Degens, G. Goldspink, and G. R. Coulton Expression of Ankrd2 in fast and slow muscles and its response to stretch are consistent with a role in slow muscle function J Appl Physiol, June 1, 2005; 98(6): 2337 - 2343. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. G. Tidball Inflammatory processes in muscle injury and repair Am J Physiol Regulatory Integrative Comp Physiol, February 1, 2005; 288(2): R345 - R353. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Glenmark, M. Nilsson, H. Gao, J.-A. Gustafsson, K. Dahlman-Wright, and H. Westerblad Difference in skeletal muscle function in males vs. females: role of estrogen receptor-{beta} Am J Physiol Endocrinol Metab, December 1, 2004; 287(6): E1125 - E1131. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| Visit Other APS Journals Online |
