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Am J Physiol Cell Physiol (December 12, 2001). doi:10.1152/ajpcell.00210.2001
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Articles in PresS, published online ahead of print December 12, 2001
Am J Physiol Cell Physiol, 10.1152/ajpcell.00210.2001
Submitted on May 7, 2001
Accepted on December 10, 2001

Excess plasma membrane and effects of ionic amphipaths on the mechanics of the outer hair cell lateral wall

Noriko Morimoto1, Robert M Raphael2, Anders Nygren3, and William E Brownell4*

1 Otolaryngology, National Children's Hospital, Tokyo, Japan
2 Bioengineering, Rice University, Hoston, TX, USA
3 Physiology and Biophysics, University of Calagary, Calgary, Alberta, Canada
4 Otorhinolaryngology and Communicative Science, Baylor College of Medicine, Houston, TX, USA

* To whom correspondence should be addressed. E-mail: brownell{at}bcm.tmc.edu.

The interaction between the outer hair cell lateral wall plasma membrane and the underlying cortical lattice was examined by a morphometric analysis of cell images during cell deformation. Vesiculation of the plasma membrane was produced by micropipette aspiration in control cells and cells exposed to ionic amphipaths that alter membrane mechanics. An increase of total cell and vesicle surface area suggests the plasma membrane possesses a membrane reservoir. Chlorpromazine (CPZ) decreased the pressure required for vesiculation while salicylate (SAL) had no effect. The time required for vesiculation was decreased by CPZ, indicating that CPZ decreases the energy barrier required for vesiculation. An increase in total volume is observed during micropipette aspiration. A deformation-induced increase in hydraulic conductivity is also seen in response to micropipette-applied fluid jet deformation of the lateral wall. Application of CPZ and/or SAL decreases this strain-induced hydraulic conductivity. The impact of ionic amphipaths on OHC plasma membrane and lateral wall mechanics may contribute to their effects on OHC electromotility and hearing.




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