Am J Physiol Cell Physiol Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Am J Physiol Cell Physiol (August 28, 2002). doi:10.1152/ajpcell.00160.2002
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
284/1/C111    most recent
00160.2002v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Rodriguez-Galan, M. C
Right arrow Articles by Correa, S. G
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Rodriguez-Galan, M. C
Right arrow Articles by Correa, S. G

Articles in PresS, published online ahead of print August 28, 2002
Am J Physiol Cell Physiol, 10.1152/ajpcell.00160.2002
Submitted on April 11, 2002
Accepted on August 23, 2002

Immunocompetence of macrophages in rats exposed to Candida albicans infection and stress

Maria C Rodriguez-Galan1, Claudia E Sotomayor1, Maria E Costamagna1, Ana M Cabanillas1, B Salido-Renteria1, Ana M Masini-Repiso1, and Silvia G Correa1*

1 Dpto Bioquimica Clinica, Universidad Nacional de Cordoba, Cordoba, Cordoba, Argentina

* To whom correspondence should be addressed. E-mail: scorrea{at}bioclin.fcq.unc.edu.ar.

A proper integration between the innate and the adaptive immune systems is required for efficient control of Candida albicans. When the homeostasis is disturbed the transition of C. albicans into an opportunistic pathogen is not uncommon and disseminated candidiasis of endogenous origin may occur. The present work aimed to assess, at the local site of the infection, the immunocompetence of macrophages in rats infected intraperitoneally with C. albicans and exposed simultaneously to stress during three days (CaS group). We studied 1) the ability to remove and kill C. albicans, 2) the TNF-{alpha} release, 3) the balance of the inducible enzymes nitric oxide synthase (iNOS) and arginase, and 4) the expression of IL-1ß and IL-1ra mRNA. Compared with only infected animals (Ca group) the number of CFU was significantly higher in CaS rats (p<0.01), and the macrophage candidicidal activity expressed as the candidicidal index was approximately 2.5 fold lower (p<0.01). The release of TNF-{alpha} was diminished in both unstimulated and heat killed C. albicans restimulated macrophages of the CaS group (Ca vs. CaS p<0.03 and p<0.05 respectively). In Ca and CaS rats both the arginase activity and the NO synthesis were significantly enhanced. However the stress exposure down regulated the activity of both enzymes (CaS vs. Ca p<0.05). After in vitro restimulation the IL-1ra/IL-1 ratio was significantly diminished in CaS rats (p<0.05). Taken together our results indicate that exists a correlation between early impairment of macrophage function and stress exposure.




This article has been cited by other articles:


Home page
 Int ImmunolHome page
M. S. Renna, S. G. Correa, C. Porporatto, C. M. Figueredo, M. P. Aoki, M. G. Paraje, and C. E. Sotomayor
Hepatocellular apoptosis during Candida albicans colonization: involvement of TNF-{alpha} and infiltrating Fas-L positive lymphocytes
Int. Immunol., December 1, 2006; 18(12): 1719 - 1728.
[Abstract] [Full Text] [PDF]

Home page
 Int ImmunolHome page
S. G. Correa, M. C. Rodriguez-Galan, B. Salido-Renteria, R. Cano, H. Cejas, and C. E. Sotomayor
High dissemination and hepatotoxicity in rats infected with Candida albicans after stress exposure: potential sensitization to liver damage
Int. Immunol., December 1, 2004; 16(12): 1761 - 1768.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
H. L. Attalah, S. Honore, S. Eddahibi, E. Marcos, C.-J. Soussy, S. Adnot, and C. Delclaux
Decreased exhaled nitric oxide as a marker of postinsult immune paralysis
J Appl Physiol, October 1, 2004; 97(4): 1188 - 1194.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Visit Other APS Journals Online
Copyright © 1976 by the American Physiological Society.