Am J Physiol Cell Physiol Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Am J Physiol Cell Physiol (August 7, 2002). doi:10.1152/ajpcell.00152.2001
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
283/6/C1627    most recent
00152.2001v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Zhong, J.
Right arrow Articles by Hume, J. R
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Zhong, J.
Right arrow Articles by Hume, J. R

Articles in PresS, published online ahead of print August 7, 2002
Am J Physiol Cell Physiol, 10.1152/ajpcell.00152.2001
Submitted on March 23, 2001
Accepted on August 5, 2002

Regulation of volume-sensitive outwardly rectifying anion channels in pulmonary arterial smooth muscle cells by protein kinase C

Juming Zhong1, Ge-Xin Wang1, William J Hatton1, Ilia A Yamboliev1, Michael P Walsh2, and Joseph R Hume1*

1 Pharmacology, University of Nevada, Reno, NV, USA; Center of Biomedical Research Excellence, University of Nevada, Reno, NV, USA
2 Biochemistry & Molecular Biology, University of Calgary, Calgary, Alberta, Canada

* To whom correspondence should be addressed. E-mail: joeh{at}med.unr.edu.

We tested the possible role of endogenous PKC in the regulation of native volume-sensitive organic osmolyte and anion channels (VSOACs) in acutely dispersed canine pulmonary artery smooth muscle cells (PASMC). Hypotonic cell swelling activated native volume-regulated Cl- currents (ICl.vol), which could be reversed by exposure to PDBu (0.1 µM), or by hypertonic cell shrinkage. Under isotonic conditions, calphostin C (0.1 µM) or Ro-31-8425 (0.1 µM), inhibitors of both conventional and novel PKC isozymes, significantly activated ICl.vol and prevented further modulation by subsequent hypotonic cell swelling. Bisindolylmaleimide (0.1 µM), a selective cPKC inhibitor, was without effect. Dialyzing acutely dispersed and cultured PASMC with {epsilon}V1-2 (10 µM), a translocation inhibitory peptide derived from the V1 region of {epsilon}PKC, activated ICl.vol under isotonic conditions and prevented further modulation by cell volume changes. Dialyzing PASMC with ßC2-2 (10 µM), a translocation inhibitory peptide derived from the C2 region of ßPKC, had no detectable effect. Immunohistochemistry in cultured canine PASMC verified that hypotonic cell swelling is accompanied by translocation of {epsilon}PKC from the vicinity of the membrane to cytoplasmic and perinuclear locations. These data suggest that membrane-bound {epsilon}PKC controls the activation state of native VSOACs in canine PASMC under isotonic and anisotonic conditions.




This article has been cited by other articles:


Home page
 J. Physiol.Home page
I. F. Abdullaev, A. Rudkouskaya, G. P. Schools, H. K. Kimelberg, and A. A. Mongin
Pharmacological comparison of swelling-activated excitatory amino acid release and Cl- currents in cultured rat astrocytes
J. Physiol., May 1, 2006; 572(3): 677 - 689.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
G.-L. Wang, G.-X. Wang, S. Yamamoto, L. Ye, H. Baxter, J. R Hume, and D. Duan
Molecular mechanisms of regulation of fast-inactivating voltage-dependent transient outward K+ current in mouse heart by cell volume changes
J. Physiol., October 15, 2005; 568(2): 423 - 443.
[Abstract] [Full Text] [PDF]

Home page
 JGPHome page
A. Zholos, B. Beck, V. Sydorenko, L. Lemonnier, P. Bordat, N. Prevarskaya, and R. Skryma
Ca2+- and Volume-sensitive Chloride Currents Are Differentially Regulated by Agonists and Store-operated Ca2+ Entry
J. Gen. Physiol., January 31, 2005; 125(2): 197 - 211.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
A. A. Mongin and H. K. Kimelberg
ATP regulates anion channel-mediated organic osmolyte release from cultured rat astrocytes via multiple Ca2+-sensitive mechanisms
Am J Physiol Cell Physiol, January 1, 2005; 288(1): C204 - C213.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
Y. Ding, D. Schwartz, P. Posner, and J. Zhong
Hypotonic swelling stimulates L-type Ca2+ channel activity in vascular smooth muscle cells through PKC
Am J Physiol Cell Physiol, August 1, 2004; 287(2): C413 - C421.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
G.-X. Wang, C. McCrudden, Y.-P. Dai, B. Horowitz, J. R. Hume, and I. A. Yamboliev
Hypotonic activation of volume-sensitive outwardly rectifying chloride channels in cultured PASMCs is modulated by SGK
Am J Physiol Heart Circ Physiol, August 1, 2004; 287(2): H533 - H544.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
G.-X. Wang, W. J. Hatton, G. L. Wang, J. Zhong, I. Yamboliev, D. Duan, and J. R. Hume
Functional effects of novel anti-ClC-3 antibodies on native volume-sensitive osmolyte and anion channels in cardiac and smooth muscle cells
Am J Physiol Heart Circ Physiol, October 1, 2003; 285(4): H1453 - H1463.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Visit Other APS Journals Online
Copyright © 1976 by the American Physiological Society.