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Am J Physiol Cell Physiol (November 12, 2003). doi:10.1152/ajpcell.00108.2003
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Submitted on March 24, 2003
Accepted on November 3, 2003

Endothelia of Schlemm's Canal and Trabecular Meshwork: Distinct Molecular, Functional and Anatomic Features

Jorge A Alvarado1*, Abigail Betanzos2, Linda Franse-Carman1, Janet Chen1, and Lorenza Gonzalez-Mariscal2

1 Ophthalmology, University of California San Francisco, San Francisco, CA, USA
2 Physiology, Biophysics, and Neuroscience, CINVESTAV, Mexico, D.F., Mexico

* To whom correspondence should be addressed. E-mail: jalva{at}itsa.ucsf.edu.

Purpose: To compare human endothelial cells from Schlemm's canal (SCEs) and the trabecular meshwork (TMEs) in terms of the ZO-1 isoform expression, hydraulic conductivity (HC) properties, and "giant" vacuole (GV) formation. Methods: The principal study methods were western blots, RT-PCR, immunofluorescence and perfusion chambers. Results: Blot signals for a+ and a- isoforms were similar in SCEs, but less intense for the a+ relative to the a- signal in TMEs. With the anti-a+ antibody used at 1/50 dilution binding occurred at cell borders of both cell types, but only to SCEs when used at a >=1/200 dilution in vitro and in vivo. SCEs were more resistive than TMEs (HC=0.66 vs. 1.32µl/min/mmHg/cm2, P<0.001) when perfused from apex to base. When perfused in the other direction, SCEs were again more resistive (5.23 vs. 9.04 units, P<0.01). GV formation occurred only in SCEs as a function of flow direction, perfusion pressure and time. Conclusion: SCEs and TMEs have distinctive phenotypic properties involving their content of ZO-1 isoforms, barrier function and GV formation.




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