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Am J Physiol Cell Physiol (September 25, 2002). doi:10.1152/ajpcell.00087.2002
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Articles in PresS, published online ahead of print September 25, 2002
Am J Physiol Cell Physiol, 10.1152/ajpcell.00087.2002
Submitted on February 28, 2002
Accepted on September 9, 2002

The Cellular Distributions of Creatine Kinase in Branchia of Euryhaline Tilapia (Oreochromis mossambicus)

Li-Yih Lin1*, Chia-Chang Chiang2, Hong-Yi Gong1, Ching-Yi Cheng2, Pung-Pung Hwang1, and Ching-Feng Weng3

1 National Defense Medical Center, Graduate Institute of Life Sciences, Taipei, Taiwan, Taiwan; Academia Sinica, Institute of Zoology, Taipei, Taiwan, Taiwan
2 Academia Sinica, Institute of Zoology, Taipei, Taiwan, Taiwan
3 National Dong Hwa University, Institute of Biotechnology, Hualien, Taiwan, Taiwan

* To whom correspondence should be addressed. E-mail: linly{at}gate.sinica.edu.tw.

Although euryhaline teleosts can adapt to environmental fluctuation of salinity, their energy source to respond to change in salinity and osmolarity remains unclear. This study examines the cellular localization of creatine kinase (CK) expression in bronchial of tilapia (Oreochromis mossambicus). Western blot analysis of muscle-type CK (MM form) revealed a high association with salinity changes; but BB and MB forms of CK in the gills of fish adapted to SW did not change. By using immunocytochemistry, three CK isoforms (MM-, MB- and BB-) were localized in mitochondria-rich (MR) cells and other epithelial cells of tilapia gills. In addition, staining intensity of MM form CK in MR cells increased after SW-transfer while BB and MB forms did not significantly change. To our knowledge, this work presents the first evidence of CK expressions in MR cells of tilapia gills, highlighting the potential role of CK providing energy for ion transport.




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[Abstract] [Full Text] [PDF]


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