Submitted on February 23, 2007
Accepted on May 25, 2007
ISOPROTERENOL INCREASES SORTING OF PAROTID GLAND CARGO PROTEINS TO THE BASOLATERAL PATHWAY.
Srirangapatnam G Venkatesh1, Jinlian Tan1, Sven-Ulrik Gorr1, and Douglas S Darling1*
1 PEDH, Oral Health and Systemic Disease, University of Louisville School of Dentistry, Louisville, Kentucky, United States
* To whom correspondence should be addressed. E-mail: douglas.darling{at}louisville.edu.
Exocrine cells have an essential function of sorting secreted proteins into the correct secretory pathway. A clear understanding of sorting in salivary glands would contribute to the correct targeting of therapeutic transgenes. The present work investigated whether there is a change in the relative proportions of basic PRP and acidic PRPs in secretory granules in response to chronic isoproterenol treatment, and whether this alters the sorting of endogenous cargo proteins. Immunoblotting of secretory granules from rat parotids found a large increase of basic PRP over acidic PRPs in response to chronic isoproterenol treatment. Pulse chase experiments demonstrated that isoproterenol also decreased regulated secretion of newly synthesized secretory proteins, including PRPs, amylase and parotid secretory protein. This decreased efficiency of the apical regulated pathway may be mediated by alkalization of the secretory granules since it was reversed by treatment with mild acid. We also investigated changes in secretion through the basolateral (endocrine) pathways. A significant increase in parotid secretory protein and salivary amylase was detected in sera of isoproterenol-treated animals suggesting increased routing of the regulated secretory proteins to the basolateral pathway. These studies demonstrate that shifts of endogenous proteins can modulate regulated secretion and sorting of cargo proteins.
