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Am J Physiol Cell Physiol (May 22, 2002). doi:10.1152/ajpcell.00079.2002
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Articles in PresS, published online ahead of print May 22, 2002
Am J Physiol Cell Physiol, 10.1152/ajpcell.00079.2002
Submitted on February 19, 2002
Accepted on May 15, 2002

Acid-base effects on intestinal Na+ absorption and vesicular trafficking

Alan N Charney1*, Richard W Egnor2, Jesline Alexander-Chacko1, Nicholas Cassai3, and Gurdip S Sidhu4

1 Nephrology, V.A. Medical Center, New York, NY, USA; Nephrology, New York University School of Medicine, New York, NY, USA
2 Nephrology, V.A. Medical Center, New York, NY, USA
3 Pathology, V.A. Medical Center, New York, NY, USA
4 Pathology, V.A. Medical Center, New York, NY, USA; Pathology, New York University School of Medicine, New York, NY, USA

* To whom correspondence should be addressed. E-mail: alan.charney{at}med.va.gov.

We examined for vesicular trafficking of NHE in pH-stimulated ileal and CO2-stimulated colonic Na+ absorption. Subapical vesicles in rat distal ileum were quantified by transmission electron microscopy at 27,500x magnification. Internalization of ileal apical membranes labeled with FITC-phytohemagglutinin was assessed using confocal microscopy. pH-stimulated ileal Na+ absorption was measured after exposure to wortmannin. Apical membrane protein biotinylation of ileal and colonic segments and Western blots of recovered proteins were performed. In ileal epithelial cells incubated in HCO3- or HEPES Ringer, the number of subapical vesicles, the relative quantity of apical membrane NHE2 and NHE3, and apical membrane fluorescence under the confocal microscope were not affected by pH between 7.1 and 7.6. Wortmannin did not inhibit pH-stimulated ileal Na+ absorption. In colonic epithelial apical membranes, NHE3 protein content was greater at Pco2 70 than 21 mmHg Pco2, was internalized when Pco2 was reduced and was exocytosed when Pco2 was increased. We conclude that vesicle trafficking plays no part in pH-stimulated ileal Na+ absorption but is important in CO2-stimulated colonic Na+ absorption.




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