Am J Physiol Cell Physiol AJP: Endocrinology and Metabolism
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Am J Physiol Cell Physiol (June 2, 2004). doi:10.1152/ajpcell.00070.2004
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
287/3/C643    most recent
00070.2004v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Puig-de-Morales, M.
Right arrow Articles by Fredberg, J. J
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Puig-de-Morales, M.
Right arrow Articles by Fredberg, J. J
Submitted on February 5, 2004
Accepted on April 13, 2004

Cytoskeletal mechanics in the adherent human airway smooth muscle cell: probe specificity and scaling of protein-protein dynamics

Marina Puig-de-Morales1*, Emil Millet1, Ben Fabry2, Daniel Navajas3, Ning Wang1, James P Butler1, and Jeffrey J Fredberg1

1 Physiology Program, Harvard School of Public Health, Boston, MA, USA
2 Physics Department, Erlangen University, Erlangen, Germany
3 Unitat Biofisica i Bioenginyeria, Facultat Medicina, Universitat Barcelona - IDIBAPS, Barcelona, Spain

* To whom correspondence should be addressed. E-mail: mpuigdem{at}hsph.harvard.edu.

We probed elastic and loss moduli in the adherent human airway smooth muscle cell through a variety of receptor systems, each serving as a different molecular window on cytoskeletal dynamics. Coated magnetic microbeads were attached to the cell surface via coating-receptor binding. A panel of bead coatings was investigated: a peptide containing the sequence RGD, vitronectin, urokinase, activating antibody against {beta}1 integrin, non-activating antibody against {beta}1 integrin, blocking antibody against {beta}1 integrin, antibody against {beta}3 integrin, and acetylated-low density lipoprotein. An oscillatory mechanical torque was applied to the bead and resulting lateral displacements were measured at baseline, after actin disruption by cytochalasin D, or after contractile activation by histamine. As expected, mechanical moduli depended strongly upon bead type and bead-coating, differing at the extremes by as much as two orders of magnitude. In every case, however, elastic and loss moduli increased with frequency, f, as a weak power law, fx-1. Moreover, with few exceptions data could be scaled such that suitably normalized elastic and frictional responses depended solely on the power law exponent x. Taken together, these data suggest that power law behavior represents a generic feature of underlying protein-protein dynamics.




This article has been cited by other articles:


Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
N. J. Fairbank, S. C. Connolly, J. D. MacKinnon, K. Wehry, L. Deng, and G. N. Maksym
Airway smooth muscle cell tone amplifies contractile function in the presence of chronic cyclic strain
Am J Physiol Lung Cell Mol Physiol, September 1, 2008; 295(3): L479 - L488.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
C. T. Mierke, P. Kollmannsberger, D. Paranhos Zitterbart, J. Smith, B. Fabry, and W. H. Goldmann
Mechano-Coupling and Regulation of Contractility by the Vinculin Tail Domain
Biophys. J., January 15, 2008; 94(2): 661 - 670.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
G. Massiera, K. M. Van Citters, P. L. Biancaniello, and J. C. Crocker
Mechanics of Single Cells: Rheology, Time Dependence, and Fluctuations
Biophys. J., November 15, 2007; 93(10): 3703 - 3713.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
M. Puig-de-Morales-Marinkovic, K. T. Turner, J. P. Butler, J. J. Fredberg, and S. Suresh
Viscoelasticity of the human red blood cell
Am J Physiol Cell Physiol, August 1, 2007; 293(2): C597 - C605.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
S. Ito, A. Majumdar, H. Kume, K. Shimokata, K. Naruse, K. R. Lutchen, D. Stamenovic, and B. Suki
Viscoelastic and dynamic nonlinear properties of airway smooth muscle tissue: roles of mechanical force and the cytoskeleton
Am J Physiol Lung Cell Mol Physiol, June 1, 2006; 290(6): L1227 - L1237.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
M. F. Coughlin, M. Puig-de-Morales, P. Bursac, M. Mellema, E. Millet, and J. J. Fredberg
Filamin-A and Rheological Properties of Cultured Melanoma Cells
Biophys. J., March 15, 2006; 90(6): 2199 - 2205.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
R. E. Laudadio, E. J. Millet, B. Fabry, S. S. An, J. P. Butler, and J. J. Fredberg
Rat airway smooth muscle cell during actin modulation: rheology and glassy dynamics
Am J Physiol Cell Physiol, December 1, 2005; 289(6): C1388 - C1395.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
L. Deng, N. J. Fairbank, D. J. Cole, J. J. Fredberg, and G. N. Maksym
Airway smooth muscle tone modulates mechanically induced cytoskeletal stiffening and remodeling
J Appl Physiol, August 1, 2005; 99(2): 634 - 641.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Visit Other APS Journals Online
Copyright © 1977 by the American Physiological Society.