| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1 Associate Professor of Department of Physiology, College of Veterinary Medicine, Gwangju, Chonnam National University, Korea, Republic of
* To whom correspondence should be addressed. E-mail: hjhan{at}chonnam.ac.kr.
Exposure of oxalate to renal proximal tubule cells may play an important role in the cell proliferation, but the signaling pathways involved in this effect have not been elucidated. Thus, the present study was performed to examine the effect of oxalate on [3H]-thymidine incorporation and its related signal pathway in primary cultured rabbit renal proximal tubule cells (PTCs). The effects of oxalate on [3H]-thymidine incorporation, LDH release, trypan blue exclusion, H2O2 release, activation of mitogen activated protein kinases (MAPKs), and [3H]-arachidonic acid (AA) release were examined in the primary cultured renal PTCs. Oxalate inhibited [3H]-thymidine incorporation in a time- and dose-dependent manner. However, its analogues did not affect in [3H]-thymidine incorporation. Oxalate (1 mM) significantly increased H2O2 release, which was blocked by N-acetylcysteine (NAC) and catalase (antioxidants). Oxalate significantly increased the p38 MAPK and stress activated protein kinase (SAPK)/c-Jun N-terminal kinase (JNK) activity, not p44/42 MAPK. Oxalate stimulated [3H]-AA release and translocation of cPLA2 from cytosolic fraction to membrane fraction. Indeed, oxalate significantly increased prostaglandin E2 production compared to control. Oxalate-induced inhibition of [3H]-thymidine incorporation and increase of [3H]-AA release were prevented by antioxidants (NAC), a p38 MAPK inhibitor (SB 203580), a SAPK/JNK inhibitor (SP 600125), or phospholipase A2 inhibitors (mepacrine and AACOCF3), but not by a p44/42 MAPK inhibitor (PD 98059). These findings suggest that oxalate inhibits renal PTC proliferation via oxidative stress, p38 MAPK/JNK, and cPLA2 signaling pathways.
This article has been cited by other articles:
|
|
 |
|
  A. Yalcin, B. F. Clem, A. Simmons, A. Lane, K. Nelson, A. L. Clem, E. Brock, D. Siow, B. Wattenberg, S. Telang, et al. Nuclear Targeting of 6-Phosphofructo-2-kinase (PFKFB3) Increases Proliferation via Cyclin-dependent Kinases J. Biol. Chem., September 4, 2009; 284(36): 24223 - 24232. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Tavolari, M. Bonafe, M. Marini, C. Ferreri, G. Bartolini, E. Brighenti, S. Manara, V. Tomasi, S. Laufer, and T. Guarnieri Licofelone, a dual COX/5-LOX inhibitor, induces apoptosis in HCA-7 colon cancer cells through the mitochondrial pathway independently from its ability to affect the arachidonic acid cascade Carcinogenesis, February 1, 2008; 29(2): 371 - 380. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Cong, Z.-L. Xiao, P. Biancani, and J. Behar Reactive oxygen species are messengers in maintenance of human and guinea pig gallbladder tonic contraction Am J Physiol Gastrointest Liver Physiol, December 1, 2007; 293(6): G1244 - G1251. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Cheng, H. U. Stotz, K. Hippchen, and A. T. Bakalinsky Genome-Wide Screen for Oxalate-Sensitive Mutants of Saccharomyces cerevisiae Appl. Envir. Microbiol., September 15, 2007; 73(18): 5919 - 5927. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. J. Han, M. J. Lim, Y. J. Lee, J. H. Lee, I. S. Yang, and M. Taub Uric acid inhibits renal proximal tubule cell proliferation via at least two signaling pathways involving PKC, MAPK, cPLA2, and NF-{kappa}B Am J Physiol Renal Physiol, January 1, 2007; 292(1): F373 - F381. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. J. Lee and H. J. Han Role of ATP in DNA synthesis of renal proximal tubule cells: involvement of calcium, MAPKs, and CDKs Am J Physiol Renal Physiol, July 1, 2006; 291(1): F98 - F106. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| Visit Other APS Journals Online |
