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Am J Physiol Cell Physiol (April 25, 2007). doi:10.1152/ajpcell.00057.2007
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Submitted on February 9, 2007
Accepted on April 18, 2007

Carbonic anhydrase XIV in skeletal muscle - subcellular localization and function from wild-type and knockout mice

Petra Wetzel1*, Renate J Scheibe2, Bernd Hellmann1, Janine Hallerdei1, Gul N Shah3, Abdul Waheed3, Gerolf Gros1, and William S Sly3

1 Zentrum Physiologie, Medizinische Hochschule Hannover, Hannover, Germany
2 Zentrum Biochemie, Medizinische Hochschule Hannover, Hannover, Germany
3 Department of Biochemistry and Molecular Biology, Saint Louis University, Saint Louis, Missouri, United States

* To whom correspondence should be addressed. E-mail: wetzel.petra{at}mh-hannover.de.

The expression of carbonic anhydrase (CA) XIV has been investigated in mouse skeletal muscles. Sarcoplasmic reticulum (SR) and sarcolemmal (SL) membrane fractions have been isolated from wild-type (WT) and CA XIV knockout (KO) mice. The CA XIV protein of 54 kDa is present in SR and SL membrane fractions as shown by Western blotting. CA activity measurements of WT and KO membrane fractions showed that CA XIV accounts for about 50% and 66% of the total CA activities determined in the SR and SL fractions, respectively. This indicates the presence of at least one other membrane-associated CA isoform, e.g. CA IV, CA IX or CA XII. Muscle fibers of the M. extensor digitorum longus (EDL) were immunostained with anti-CA XIV/FITC and anti-SERCA 1/TRITC or with anti-CA XIV/FITC and anti-RyR/TRITC or with anti-CA XIV/FITC and anti-MCT 4/TRITC. CA XIV is expressed in the plasma membrane and in the longitudinal SR, but not in the terminal SR. Isometric contraction measurements of single twitches and tetani and a fatigue protocol applied to fiber bundles of the fast-twitch EDL and of the slow-twitch M. soleus (SOL) from WT and KO mice showed that the lack of SR membrane-associated CA XIV did not affect maximum force, rise and relaxation times, and fatigue behavior. Thus, it is concluded that a reduction of the total SR-CA activity by about 50% in the CA XIV KO mice does not lead to an impairment of SR function.




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R. J. Scheibe, K. Mundhenk, T. Becker, J. Hallerdei, A. Waheed, G. N. Shah, W. S. Sly, G. Gros, and P. Wetzel
Carbonic anhydrases IV and IX: subcellular localization and functional role in mouse skeletal muscle
Am J Physiol Cell Physiol, February 1, 2008; 294(2): C402 - C412.
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