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Am J Physiol Cell Physiol (March 24, 2004). doi:10.1152/ajpcell.00054.2004
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Submitted on January 27, 2004
Accepted on March 20, 2004

Changes in Gene Expression in the Gills of the Euryhaline Killifish Fundulus heteroclitus After Abrupt Salinity Transfer

Graham R Scott1*, Jeff G Richards1, Biff Forbush2, Paul Isenring3, and Patricia M Schulte1

1 Zoology, University of British Columbia, Vancouver, BC, Canada
2 Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, CT, USA; Mount Desert Island Biological Laboratory, Salsbury Cove, MN, USA
3 Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, CT, USA; Mount Desert Island Biological Laboratory, Salsbury Cove, MN, USA; Groupe de Recherche en Nephrologie, Department of Medicine, Laval University, Laval, PQ, Canada

* To whom correspondence should be addressed. E-mail: scott{at}zoology.ubc.ca.

Maintenance of ion balance requires that ionoregulatory epithelia modulate ion flux in response to internal or environmental osmotic challenges. We have explored the basis of this functional plasticity in the gills of the euryhaline killifish Fundulus heteroclitus. The expression patterns of several genes encoding ion transport proteins were quantified after transfer from near-isosmotic brackish water (10 ppt) to either freshwater (FW) or seawater (SW). Many changes in response to SW transfer were transient. Increased mRNA expression occurred 1 day after transfer for Na+,K+-ATPase {alpha}1a (3-fold), Na+,K+,2Cl--cotransporter 1 (NKCC1) (3-fold), and glucocorticoid receptor (1.3-fold), and was paralleled by elevated Na+,K+-ATPase activity (2-fold). The transient increase in NKCC1 mRNA expression was followed by a later 2-fold rise in NKCC protein abundance. In contrast to the other genes studied here, mRNA expression of the cystic fibrosis transmembrane conductance regulator (CFTR) Cl- channel generally remained elevated (2-fold) in SW. No change in protein abundance was detected, however, suggesting post-transcriptional regulation. The responses to FW transfer were quite different than to SW transfer. In particular, FW transfer increased Na+,K+-ATPase {alpha}1a mRNA expression and Na+,K+-ATPase activity to a greater extent than SW transfer, but had no effect on V-type H+-ATPase expression, supporting the current suggestion that killifish gills transport Na+ via Na+,H+-exchange. These findings demonstrate unique patterns of ion transporter expression in killifish gills after salinity transfer, and illustrate important mechanisms of functional plasticity in ion transporting epithelia.




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