| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Articles in PresS, published online ahead of print April 24, 2002
Am J Physiol Cell Physiol, 10.1152/ajpcell.00054.2002
Submitted on February 4, 2002
Accepted on April 18, 2002
1 Surgery, University of Maryland School of Medicine, Baltimore, MD, USA
* To whom correspondence should be addressed. E-mail: jwang{at}smail.umaryland.edu.
Cellular polyamines are essential for early mucosal restitution that occurs by epithelial cell migration to reseal superficial wounds after injury. Normal intestinal epithelial cells are tightly bound in sheets, but they need to be rapidly disassembled during restitution. ß-catenin is involved in cell-cell adhesion and its tyrosine phosphorylation causes disassembly of adhesion junctions, enhancing the spreading of cells. The current study was designed to test the hypothesis that polyamines are required for the stimulation of intestinal epithelial cell migration during restitution by altering beta-catenin tyrosine phosphorylation. Migration of intestinal epithelial cells (IEC-6 line) after wounding was associated with a significant increase in ß-catenin tyrosine phosphorylation, which decreased the binding activity of ß-catenin to ß-catenin. Depletion of cellular polyamines by 
-difluoromethylonithine (DFMO) reduced cytoplasmic free Ca2+ concentration ([Ca2+]cyt), prevented the induction of ß-catenin phosphorylation, and decreased cell migration. Elevation of [Ca2+]cyt induced by the Ca2+ ionophore ionomycin restored ß-catenin phosphorylation and promoted migration in polyamine-deficient cells. Decreased ß-catenin phosphorylation through the tyrosine kinase inhibitor herbimycin-A or genistein blocked cell migration, which was accompanied by reorganization of cytoskeletal proteins, along with the heavy actin cortex around the entire cells. These results indicate that ß-catenin tyrosine phosphorylation plays a critical role in polyamine-dependent cell migration and that polyamines induce ß-catenin tyrosine phosphorylation at least partially through [Ca2+]cyt.
This article has been cited by other articles:
|
|
 |
|
  N. N. Raveendran, K. Silver, L. C. Freeman, D. Narvaez, K. Weng, S. Ganta, and J. D. Lillich Drug-Induced Alterations to Gene and Protein Expression in Intestinal Epithelial Cell 6 Cells Suggest a Role for Calpains in the Gastrointestinal Toxicity of Nonsteroidal Anti-Inflammatory Agents J. Pharmacol. Exp. Ther., May 1, 2008; 325(2): 389 - 399. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Guo, J. W. Breslin, M. H. Wu, C. J. Gottardi, and S. Y. Yuan VE-cadherin and {beta}-catenin binding dynamics during histamine-induced endothelial hyperpermeability Am J Physiol Cell Physiol, April 1, 2008; 294(4): C977 - C984. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. N. Rao, O. Platoshyn, V. A. Golovina, L. Liu, T. Zou, B. S. Marasa, D. J. Turner, J. X.-J. Yuan, and J.-Y. Wang TRPC1 functions as a store-operated Ca2+ channel in intestinal epithelial cells and regulates early mucosal restitution after wounding Am J Physiol Gastrointest Liver Physiol, April 1, 2006; 290(4): G782 - G792. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Guo, J. N. Rao, L. Liu, T. Zou, K. M. Keledjian, D. Boneva, B. S. Marasa, and J.-Y. Wang Polyamines are necessary for synthesis and stability of occludin protein in intestinal epithelial cells Am J Physiol Gastrointest Liver Physiol, June 1, 2005; 288(6): G1159 - G1169. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Liu, R. Santora, J. N. Rao, X. Guo, T. Zou, H. M. Zhang, D. J. Turner, and J.-Y. Wang Activation of TGF-{beta}-Smad signaling pathway following polyamine depletion in intestinal epithelial cells Am J Physiol Gastrointest Liver Physiol, November 1, 2003; 285(5): G1056 - G1067. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Guo, J. N. Rao, L. Liu, T.-T. Zou, D. J. Turner, B. L. Bass, and J.-Y. Wang Regulation of adherens junctions and epithelial paracellular permeability: a novel function for polyamines Am J Physiol Cell Physiol, November 1, 2003; 285(5): C1174 - C1187. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. M. Martin, A. E. Sutherland, and L. J. Van Winkle Amino Acid Transport Regulates Blastocyst Implantation Biol Reprod, October 1, 2003; 69(4): 1101 - 1108. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Piedra, S. Miravet, J. Castano, H. G. Palmer, N. Heisterkamp, A. Garcia de Herreros, and M. Dunach p120 Catenin-Associated Fer and Fyn Tyrosine Kinases Regulate {beta}-Catenin Tyr-142 Phosphorylation and {beta}-Catenin-{alpha}-Catenin Interaction Mol. Cell. Biol., April 1, 2003; 23(7): 2287 - 2297. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. N. Rao, X. Guo, L. Liu, T. Zou, K. S. Murthy, J. X.-J. Yuan, and J.-Y. Wang Polyamines regulate Rho-kinase and myosin phosphorylation during intestinal epithelial restitution Am J Physiol Cell Physiol, April 1, 2003; 284(4): C848 - C859. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| Visit Other APS Journals Online |
