Submitted on January 31, 2007
Accepted on May 24, 2007
Mitochondrial alterations in human gastric carcinoma cell line
Hyoung Kyu Kim1, Won Sun Park1, Sung Hyun Kang1, Mohamad Warda1, Nari Kim1, Jae-Hong Ko1, and Jin Han1*
1 Department of Physiology and Biophysics, College of Medicine, Inje University, Busan, Korea, Republic of
* To whom correspondence should be addressed. E-mail: Phyhanj{at}inje.ac.kr.
We compared mitochondrial function, morphology, and proteome in the rat normal gastric cell line RGM-1 and the human gastric cancer cell line AGS. The total number and cross-sectional size of mitochondria were smaller in AGS cells. The mitochondria in AGS cells were deformed and consumed less oxygen. Confocal microscopy indicated that the mitochondrial inner membrane potential was hyperpolarized and the mitochondrial Ca2+ concentration were elevated in AGS. Interestingly, two-dimensional electrophoresis proteomics on the mitochondria-enriched fraction revealed high expression of four mitochondrial proteins in AGS: ubiquinol-cytochrome c reductase, mitochondrial short-chain enoyl-coenzyme A hydratase-1, heat shock protein 60, and mitochondria elongation factor TU in AGS. The results provide clues as to the mechanism of the mitochondrial changes in cancer at the protein level and may serve as potential cancer biomarker in mitochondria.
