| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1 Immunology, Baylor College of Medicine, Houston, Texas, United States; Pediatrics, Baylor College of Medicine, Houston, Texas, United States
2 Pediatrics, Baylor College of Medicine, Houston, Texas, United States
3 Pediatrics-Nutrition, USDA/ARS, Houston, Texas, United States; Pediatrics, Baylor College of Medicine, Houston, Texas, United States
4 Departments of Pediatrics, Immunology, and Medicine, Baylor College of Medicine, Houston, Texas, United States
5 School of Pediatrics and Reproductive Health, University of Adelaide, Adelaide, South Australia, Australia
* To whom correspondence should be addressed. E-mail: cwsmith{at}bcm.tmc.edu.
Obesity has been linked to cardiovascular disease, hypertension, diabetes and metabolic syndrome with elevated markers of systemic inflammation. Intercellular adhesion molecule-1 (ICAM-1) is a transmembrane adhesion molecule involved in leukocyte migration to sites of inflammation. In human obesity elevations in the soluble form of ICAM-1 (sICAM-1) are positively correlated with abdominal fat deposition. Increases in adiposity have also been correlated with macrophage infiltration into adipose tissue. Here we investigate adipose tissue production and transcriptional regulation of ICAM-1 in a mouse model of dietary obesity. After feeding a high fat diet, ICAM-1 in serum and adipose tissue was analysed by ELISA, Northern blot, real-time quantitative PCR, and flow cytometry. After 6 months on the high fat diet, sICAM-1 levels significantly correlated with body weight and abdominal fat mass. ICAM-1 mRNA was expressed in adipose tissue of mice with significantly higher levels in males than females. After only 3 weeks there was an adipose tissue-specific increase in mRNA for ICAM-1, IL-6 and MCP-1 in male mice. Analysis of the stromal vascular fraction of male adipose tissue revealed CD11b negative cells with increased surface ICAM-1 and CD34. We also found two populations of F4/80+, CD11b+, ICAM-1+ cells, one of which also expressed CD14 and CD11c and was increased in response to a high fat diet. These results indicate that within 3 weeks on a high fat diet male mice exhibited significant increases in pro-inflammatory factors and immune cell infiltration in adipose tissue that may represent links between obesity and its associated inflammatory complications.
This article has been cited by other articles:
|
|
 |
|
  G. Musso, R. Gambino, S. Bo, and M. Cassader Should Nonalcoholic Fatty Liver Disease Be Included in the Definition of Metabolic Syndrome? A Cross-Sectional Comparison With Adult Treatment Panel III Criteria in Nonobese Nondiabetic Subjects: Response to Sookoian et al. Diabetes Care, May 1, 2008; 31(5): e43 - e43. [Full Text] [PDF]
|
|
|
|
 |
|
 M. Lorenzo, S. Fernandez-Veledo, R. Vila-Bedmar, L. Garcia-Guerra, C. De Alvaro, and I. Nieto-Vazquez Insulin resistance induced by tumor necrosis factor-{alpha} in myocytes and brown adipocytes J Anim Sci, April 1, 2008; 86(14_suppl): E94 - E104. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. J. Strissel, Z. Stancheva, H. Miyoshi, J. W. Perfield II, J. DeFuria, Z. Jick, A. S. Greenberg, and M. S. Obin Adipocyte Death, Adipose Tissue Remodeling, and Obesity Complications Diabetes, December 1, 2007; 56(12): 2910 - 2918. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. R. Coenen, M. L. Gruen, A. Chait, and A. H. Hasty Diet-Induced Increases in Adiposity, but Not Plasma Lipids, Promote Macrophage Infiltration Into White Adipose Tissue Diabetes, March 1, 2007; 56(3): 564 - 573. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| Visit Other APS Journals Online |
