Am J Physiol Cell Physiol Ad Instruments
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Am J Physiol Cell Physiol (June 16, 2004). doi:10.1152/ajpcell.00004.2004
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
287/4/C1048    most recent
00004.2004v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (14)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Garcia, K. D
Right arrow Articles by Garcia, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Garcia, K. D
Right arrow Articles by Garcia, J.
Submitted on January 6, 2004
Accepted on June 14, 2004

Immunolocalization of Type 2 Inositol 1,4,5-Trisphosphate Receptors in Cardiac Myocytes from Newborn Mice

Kelly D Garcia1, Tanvi Shah2, and Jesus Garcia2*

1 Research Services, Edward Hines Jr. VA Hospital, Hines, IL, USA
2 Physiology and Biophysics, University of Illinois at Chicago, Chicago, IL, USA

* To whom correspondence should be addressed. E-mail: garmar{at}uic.edu.

The precise localization and role of InsP3Rs in cardiac muscle cells are largely unknown. It is believed that waves and oscillations in cytosolic free calcium triggered by activation of InsP3Rs underlie modifications of cellular responses that lead to changes in gene expression in other cells. However, how changes in cytosolic calcium alter gene expression in cardiac cells is unknown. Moreover, it is unclear how changes in cytosolic calcium that alter gene expression do so independently of effects of calcium on other cellular functions, such as contraction. Here we show that InsP3R type 2 is the only isoform present in cardiac myocytes isolated from neonatal mouse ventricules. We also show that InsP3Rs-2 are associated with the nucleus and that activation of InsP3Rs-2 with endothelin-1 or phenylephrine selectively increases transcription of atrial natriuretic factor and skeletal {alpha}-actin. InsP3Rs-2 are also in striations. Activation of InsP3Rs with Adenophostin-A in permeabilized cells induced calcium release in the nuclear domain and other regions of the cell away from the nucleus. Agonist-induced increase in gene expression and calcium release were blocked by the InsP3Rs inhibitors 2-aminoethoxydiphenyl borate or XestosponginC. The spatial separation of InsP3Rs-2 provides support that microdomains of calcium discretely alter various cell processes. Our experiments suggest that calcium released by InsP3Rs-2 in the nuclear domain provides a direct mechanism for the control of gene expression whereas release of calcium in the cytoplasm may modulate other processes, such as contraction.




This article has been cited by other articles:


Home page
 J. Cell Sci.Home page
M. D. Bootman, C. Fearnley, I. Smyrnias, F. MacDonald, and H. L. Roderick
An update on nuclear calcium signalling
J. Cell Sci., July 15, 2009; 122(14): 2337 - 2350.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
A. V. Zima, D. J. Bare, G. A. Mignery, and L. A. Blatter
IP3-dependent nuclear Ca2+ signalling in the mammalian heart
J. Physiol., October 15, 2007; 584(2): 601 - 611.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
K. Przyklenk, M. Maynard, and P. Whittaker
First molecular evidence that inositol trisphosphate signaling contributes to infarct size reduction with preconditioning
Am J Physiol Heart Circ Physiol, October 1, 2006; 291(4): H2008 - H2012.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
A. A. Lanzafame, L. Turnbull, F. Amiramahdi, J. F. Arthur, H. Huynh, and E. A. Woodcock
Inositol phospholipids localized to caveolae in rat heart are regulated by {alpha}1-adrenergic receptors and by ischemia-reperfusion
Am J Physiol Heart Circ Physiol, May 1, 2006; 290(5): H2059 - H2065.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Visit Other APS Journals Online
Copyright © 1977 by the American Physiological Society.