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CORRIGENDUM

Corrigendum

Quesnell RR, Erickson J, and Schultz BD. Apical electrolyte concentration modulates barrier function and tight junction protein localization in bovine mammary epithelium. Am J Physiol Cell Physiol 292: C305–C318. First published August 2, 2006; doi: 10.1152/ajpcell.00567.2005; http://ajpcell.physiology.org/cgi/content/full/292/1/C305.

On pages C310 through C313, Figs. 4–7 were inadvertantly printed in black and white/grayscale instead of color. A corrected version of the PDF, with the color images, is available as a link from the article online. Also, the color version of the figures is presented here, with the original legends.

View larger version (116K): [in this window] [in a new window]   Fig. 4. Zonula occludens (ZO)-1 and occludin immunoreactivity circumscribes all cells in the BME-UV cell monolayer. The image of a single focal plane from a trilabeled monolayer is presented. All cells are circumscribed by occludin (A) and ZO-1 (B) immunoreactivity that can be readily superimposed (D). That each cell is circumscribed becomes apparent by visualizing nuclei with TO-PRO3 (nuclear stain) (C) and observing that each nucleus is surrounded by immunoreactivity (D). Results are typical of 5 separate experiments.

View larger version (53K): [in this window] [in a new window]   Fig. 5. No differences in localization of ZO-1 immunoreactivity were identified in confocal images of anti-ZO-1/rhodamine-labeled cells in 14-day, L-elec medium-exposed monolayers (A) compared with 14-day, H-elec medium-exposed monolayers (B). Labeling by secondary antibody in the absence of primary antibody as well as TO-PRO-3 nuclear stain were included in (C) to demonstrate the specificity of the secondary antibody association with the primary antibody and to preclude any concerns about nonspecific secondary antibody labeling.

View larger version (208K): [in this window] [in a new window]   Fig. 7. Occludin localization is changed rapidly after apical medium changes. A: Changes in occludin localization were evident as early as 24 h after exposure to high-electrolyte apical medium. C and D: more punctuate or diffuse labeling of occludin was evident (as compared with the correlating image in Fig. 6A). B: after 7-days exposure to high-electrolyte medium in the apical compartment, occludin localization was similar to 14-day, high-electrolyte medium images (see Fig. 6B), where there is no indication of accumulation at tight junctions. C: occludin appears to begin reappearing at the level of the tight junctions by 4 days of exposure to low-electrolyte medium. D: occludin is organized at the level of the tight junctions after 7 days of exposure to low-electrolyte apical medium. Images in Figs. 6 and 7 represent cell monolayers exposed to experimental treatments in tightly paired units and processed in parallel. Confocal images represent sequential planes taken at 1-µm intervals from apical (top left) to basal (bottom right) aspects through the cell monolayer in each set of images. Results are typical of 5 separate experiments. Secondary-antibody-only controls, to rule out evidence for nonspecific labeling, for each monolayer are included as Supplementary Data (included in the online version of this article), and include goat anti-rabbit Alexa 488 secondary antibody (for occludin) and goat-anti-rat rhodamine secondary antibody (for ZO-1) treatments. TO-PRO3 nuclear staining demonstrates cell locale, as does the superimposed brightfield image. Panels at the top and right represent a horizontal (top) and vertical (right) slice through the monolayer from apical to basolateral aspects at the indicated red or green line on the image. Scale bar indicates 20 µm.

View larger version (106K): [in this window] [in a new window]   Fig. 6. Localization of occludin immunoreactivity is modified by apical media composition as shown in stacked confocal microscope images of anti-occludin/Alexa 488-labeled cells in 14-day, L-elec medium-exposed monolayers (A) compared with 14-day, H-elec medium-exposed monolayers (B). After a 2 wk exposure to L-elec medium in the apical compartment (A), occludin immunoreactivity clearly circumscribes all cells in the monolayer. After a 2 wk exposure to H-elec medium in the apical compartment, minimal occludin localization is evident at the expected level of the tight junctions compared with monolayers exposed to the low apical electrolyte concentration. Monolayers were exposed to experimental treatments in tightly paired units (in conjunction with corresponding images in Fig. 7)and processed in parallel. Confocal images represent sequential planes taken at 1-µm intervals from apical (top left) to more basolateral (bottom right) aspects through the cell monolayer. Results are typical of 5 separate experiments. Panels at top and right represent a horizontal (top) and a vertical (right) slice through the monolayer from apical to basolateral aspects at the indicated red or green line on the image. Scale bar indicates 20 µm.

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