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INVITED REVIEW
Departments of 1Surgery, 2Urology, and 3Obstetrics and Genecology, University of Michigan, Ann Arbor, Michigan
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ABSTRACT |
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migration; immune suppression; tumor angiogenesis; tumor metastasis; stem cells
and SDF-1
, from a single gene that encodes two splice variants. The two encoded proteins are almost identical, except for the last four amino acids of SDF-1, which are absent in SDF-1. Biological and functional differences between the CXCL12 isoforms have not been described. The CXCL12 gene is mapped in chromosome 10, whereas most of the other genes encoding CXC chemokines reside on chromosome 4 (126). It was long thought that CXCL12 bound exclusively to CXCR4 and that CXCR4 was its sole receptor. However, CXCR7 was identified as another receptor for CXCL12 at the end of 2005 (4). The immunological activities of CXCL12/CXCR4 have been largely studied in the context of immune cell trafficking. Interestingly, both CXCL12 and CXCR4 knockout mice are embryonic lethal, with any surviving pups dying within an hour of birth, suggesting that the CXCL12/CXCR4 pathway mediates multiple biological activities (93, 132). The lethal effect of CXCL12 and CXCR4 knockout is related to the pleiotropic activity of CXCL12 and CXCR4, which are critical for hematopoietic, neural, vascular, and craniofacial organogenesis (80, 93, 132).
In this review, we focus on the pathological nature and characteristics of CXCL12 in the tumor microenvironment. We discuss therapeutic strategies of targeting the CXCL12/CXCR4 pathway in the treatment of human cancer.
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EXPRESSION OF CXCL12 IN HUMAN TUMOR ENVIRONMENT |
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REGULATION OF CXCL12 EXPRESSION IN HUMAN TUMOR |
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CXCL12, TUMOR PROLIFERATION, AND SURVIVAL |
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In addition to glioma cells, CXCL12 can induce proliferation of several tumor cell lines, including ovarian carcinoma (120), small cell lung cancer (100), prostate cancer(23), neck squamous cell carcinoma (58), and pancreatic cancer (81). Mechanistically, CXCL12-dependent cell proliferation is linked to ERK activation (23, 60, 81, 100, 120).
CXCL12/CXCR4-mediated tumor cell proliferation may be regulated through estrogen signaling (39). About 60% of human ovarian and breast cancers are hormone dependent and overexpress the progesterone and/or estrogen receptors (55, 77). Hall et al. (39) demonstrated that CXCL12 was required for estrogen-induced proliferation of both breast and ovarian cancers.
CXCL12 also can regulate tumor cell apoptosis. CXCL12 activates NF-
B (45), which in turn inhibits radiation-induced tumor necrosis factor- (TNF-) production and tumor apoptosis (140). Moreover, activation of NF-B can sensitize cancer cells to CXCL12 stimulation through upregulation of CXCR4 expression (45, 70).
Many chemotherapeutic drugs exert their effects by inducing apoptosis in the targeted cell population. CXCL12 can protect tumor cells from drug-induced apoptosis directly through the activation of antiapoptotic pathways but also indirectly by modulating the adherence of cancer cells. For example, CXCL12 mediates adhesion of small-cell lung cancer cells (SCLC) to marrow stroma cells and protects SCLC against etoposide-induced apoptosis. The protective effect could be antagonized by CXCR4-specific inhibitors as well as by blocking integrin 4 (41, 124). Similar observations are found in myeloma (44), glioma cells (138), and head and neck cancer (91). In support of this observation, CXCL12 activates integrin 4 on vascular endothelial cells and protects plasmacytoid dendritic cell from apoptosis in patients with ovarian cancer (156). Thus CXCL12 signals may be implicated in tumor cell proliferation and survival.
The role of CXCL12 in controlling tumor growth and survival has been demonstrated in in vitro models. However, in some cases, the in vitro observations are not fully supported by in vivo experimental data. For example, glioma cells proliferate in vitro in response to CXCL12 (6); however, they proliferate in vivo independently of CXCL12 (106). Furthermore, tumor cells exhibit low proliferation in glioblastoma tissues, where high levels of CXCL12 expression are observed. Analysis of CXCR4/CXCL12 localization revealed an association of both CXCL12 and CXCR4 with regions of necrosis and angiogenesis (106), suggesting a role of CXCL12 in angiogenesis in vivo.
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CXCL12 AND TUMOR VASCULARIZATION |
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CXCL12 is an ELR– CXC chemokine; however, it exhibits angiogenic activity. Initially, the angiogenic role of CXCL12 was observed in mice lacking CXCL12 or CXCR4 (80, 132). These mice had defective formation of large vessels supplying the gastrointestinal tract. Subsequent in vitro studies suggested a potential effect of CXCL12 on blood vessel formation. For example, CXCL12 stimulates the formation of capillary-like structures with human vascular endothelial cells (83, 86, 109). Interestingly, although high concentrations of CXCL12 are able to induce angiogenesis in vivo (83), our studies have shown that pathological levels of CXCL12 alone failed to induce meaningful vascularization in vivo. However, pathological concentrations of CXCL12 induced potent neoangiogenesis in vivo in the presence of low concentrations of vascular endothelial growth factor (VEGF) (66), revealing profound synergistic effects between CXCL12 and VEGF. Furthermore, CXCL12 attracts plasmacytoid dendritic cells (DCs) into the tumor environment, and in turn tumor plasmacytoid DCs induce neoangiogenesis through production of IL-8 and TNF- (21). Therefore, a multifactor model is proposed to explain the mechanism whereby CXCL12 induces vascularization (109, 147). In this model, CXCL12 synergizes with soluble factors, including fibroblast growth factor (FGF) family members and VEGF (66, 92), and coordinates with immune cells, including plasmacytoid DCs (21), to induce potent vascularization in vivo.
Migration, expansion, and survival of vascular endothelial cells form the essential functional network of angiogenesis. Vascular endothelial cell migration is strongly dependent on CXCL12 (37, 109). In support of this, neutralizing antibodies against CXCL12 inhibit endothelial cell invasion into subcutaneously injected Matrigel (111). Hypoxia simultaneously stimulates CXCR4 expression (16, 129) and CXCL12 (66) production. Therefore, it is reasoned that hypoxia would promote vascular endothelial cell migration toward CXCL12 and induce tumor vascularization in a CXCL12-dependent manner.
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CXCL12 AND TUMOR METASTASIS |
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CXCL12 and Tumor Cell Adhesion Cancer dissemination can be viewed as a tissue remodeling process that involves proteolytic degradation of extracellular matrix. Metalloproteases (MMPs) are a family of enzymes involved in the degradation of extracellular matrix in the surrounding normal tissue and known to mediate cancer invasion and metastases (28). Activation of MMPs breaks down the physical barriers of metastasis, thus promoting invasion by cancer cells (49). Several studies have documented that CXCL12 induces MMP synthesis in different cell types (62, 75, 81, 127, 148) and facilitates tumor cell adhesion and colonization.
CXCL12 also modulates the expression and function of cell surface integrin molecules and, in turn, promotes tumor cell adhesion. Integrins are a large family of heterodimeric transmembrane glycoproteins that attach cells to extracellular matrix proteins of the basement membrane or to ligands on other cells. CXCL12 induces adhesion of SCLCs to VCAM-1, fibronectin, and collagen (13, 41).
CXCL12 and Tumor Cell Migration The CXCL12/CXCR4 pathway is involved in the "homing" of lymphocytes. It was hypothesized that chemokines and chemokine receptors including CXCL12/CXCR4 might mediate cancer cells to "home" to specific secondary sites, thereby promoting organ-specific metastasis. In 2001, Muller et al. (90) provided the first evidence that the CXCL12/CXCR4 pathway mediates human breast cancer metastasis. In vivo blocking of CXCR4 with the use of a specific antibody (90) or selective synthetic polypeptide (73) or siRNA (74) resulted in significant inhibition of breast cancer metastasis to regional lymph nodes as well as in the lung. In the presence of neutralizing CXCL12 antibodies, NSCLC tumor metastases also were significantly reduced (100). Blocking of CXCR4 expression on the cell surface greatly reduced the ability of colon cancer cells to metastasize to the liver and lungs (150). Furthermore, antibody-mediated neutralization of CXCR4 was found to limit skeletal metastasis in prostate cancer (130). Induction of CXCR4 expression resulted in a dramatic increase in pulmonary metastases of melanoma cells, a situation that could be blocked using potent CXCR4 inhibitors. Subcutaneously injected prostate cancer cells transfected with CXCR4 grew larger tumors with increased muscle invasion compared with parental cells (23). In further support of the role of CXCL12 in tumor metastasis, high levels of CXCL12 are often found in lymph nodes, lung, liver, and bone marrow, where tumors frequently metastasize (90, 100, 101, 130). Nonetheless, although the molecular mechanism of action has yet to be established, these studies demonstrate the pivotal role of CXCL12/CXCR4 in tumor metastasis.
Hypoxia induces CXCR4 expression on tumor cells (54, 117), which would sensitize tumor cells to CXCL12 signals and promote tumor metastasis. However, hypoxia simultaneously stimulates both CXCR4 and CXCL12 expression (66). Human cancer cells including neuroblastoma (32), glioblastoma (6, 106), ovarian (66, 120, 156), breast (3, 57), colon (54), pancreas (64), and prostate (131) express CXCL12 (Table 1). It is reasoned that endogenous CXCL12, together with CXCR4 on tumor cells, should keep cancer cells within the primary tumor environment, rather than facilitate metastasis over a long distance. Nonetheless, the effects of CXCL12/CXCR4 on tumor metastasis may be explained by multiple factors in the tumor environment.
Heterogeneous CXCL12 expression in different tumors. Ovarian cancers constitutively express high levels of CXCL12 (65, 66, 156). CXCL12 expression may be restricted to either the metastatic lesion or limited fragments of primary tumor.
CXCL12 sensitivity. The uncoupling of CXCR4 following receptor internalization by endocytosis may persist even after the receptor is recycled to the cell surface (125). Tumor cells by themselves also can modulate their sensitivity to the CXCL12 by regulating CD26 expression. CD26 (known as dipeptidyl peptidase IV, DPPIV) is a ubiquitously expressed 110-kDa membrane-bound extracellular peptidase and has a variety of roles in the development of human malignancies as well as in normal T cell biology. Absence of CD26 peptidase activity enhances HSC cell migration toward a CXCL12 gradient in vitro. Moreover, removal of endogenous CD26 on donor HSCs has been demonstrated to increase homing and engraftment of HSCs in vivo (18). Mizokami et al. (85) demonstrated that expression of CD26/DPPIV in endometrial carcinoma can directly modulate CXCL12 functions. CD26 is expressed on the surface of normal epithelial cells, but it is often lost in various cancers (143, 144). Hypoxia can upregulate CD26 expression on tumor cells (114) and, in turn, could decrease the sensitivity of tumor cells to local CXCL12.
Tumor cells moving away from high local levels of CXCL12. Although the mechanism remains to be defined, antigen-induced T-cell recruitment into the peritoneal cavity can be reversed by high but not low concentrations of CXCL12 (104). Analogously, one may speculate that high levels of CXCL12 in the tumor environment would trigger tumor cells migrating away from primary tumor and foster tumor metastasis.
CXCL12 is not the exclusive chemokine that regulates tumor cell trafficking. In summary, although other factors need to be considered, it is evident that the CXCL12/CXCR4 pathway is implicated in the mechanistic process of tumor metastasis, including tumor cell adhesion and migration.
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CXCL12 AND CANCER STEM CELLS |
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CXCL12 AND TUMOR IMMUNOSUPPRESSION |
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CXCL12 and CD4+CD25+ regulatory T cells. Bone marrow is a common site for human tumor metastasis, suggesting that bone marrow may provide an immunosuppressive environment for tumor retention and growth. Interestingly, a number of reports have demonstrated that functional memory T cells exist in bone marrow (7, 82). Bone marrow can serve as a site for naive tumor-associated antigen (TAA)-specific T cell priming (7, 31, 82, 136). Indeed, TAA-specific T cells isolated from the bone marrow of tumor-bearing mice and cancer patients are functional in vitro and are able to prevent tumor growth when transferred to another host. These data suggest that these TAA-specific T cells are functionally suppressed in the bone marrow (30, 31, 82, 136). This notion was supported by our recent observation that large numbers of functional CD4+ regulatory T (Treg) cells accumulate in the bone marrow of healthy volunteers and mice (153). This observation was confirmed in a FOXP3 bicistronic reporter knock-in mouse model (139). In this model, a bicistronic reporter expressing a red fluorescent protein was knocked into the endogenous FOXP3 locus. High levels of FOXP3-expressing T cells (with red fluorescence) were found in the bone marrow (139).
Strikingly, bone marrow CD4+ Treg cells express functional CXCR4, and CD4+ Treg cell release from bone marrow is achieved through granulocyte-colony-stimulating factor reducing marrow expression of CXCL12 (153). Activation of Treg cells upregulates CXCR4 expression and enables them to migrate to the bone marrow in a CXCL12-dependent manner (153), suggesting that bone marrow could serve as a functional reservoir for activated Treg cells. Thus CXCR4/CXCL12 signals are crucial for bone marrow trafficking of activated CD4+ Treg cells. High levels of Treg cells in the bone marrow may provide an immune shield to facilitate bone marrow metastasis. Therefore, CXCL12 may contribute to tumor bone marrow metastasis by recruiting Treg cells.
CXCL12 and plasmacytoid DCs. Functional plasmacytoid DCs are found in the tumor environment of patients with ovarian cancer (156), melanoma (110), and head and neck squamous cell carcinoma (HNSCC) (40). Tumor cells produce CXCL12 and plasmacytoid DCs express VLA-5 and CXCR4, the key molecules that mediate plasmacytoid DC tumor trafficking (156). CXCL12 further protects tumor plasmacytoid DCs from apoptosis (156). Strikingly, tumor-associated plasmacytoid DCs induce significant IL-10 production by T cells that suppresses myeloid DC-induced TAA-specific T cell effector functions (156). Tumor plasmacytoid DCs induced IL-10+CCR7+CD8+ T cells to home to the draining lymph nodes and suppress TAA-specific central priming (142). The fact that allogeneic plasmacytoid DCs are able to induce CD4+ (89) and CD8+ (34) suppressive regulatory T cells supports these data. A large amount of plasmacytoid DCs, but not functional mature myeloid DCs, accumulate in the tumor environment (156).
Notably, it has been shown in mouse models that locally accumulated CXCL12 can lead to the inhibition of tumor growth by facilitating the chemoattraction of leukocytes into the tumor area. In this respect, mouse leukemia and melanoma cells transfected with CXCL12 were rejected following injection into syngeneic mice (26). CXCL12 produced by melanoma cells attracts cytotoxic T cells through their binding to CXCR4. Blockade of CXCL12/CXCR4 signal inhibits CTL migration toward tumor cells (151). Likewise, depending on circumstances, it is possible that CXCL12 stimulates T cell immunity against a tumor. However, CXCL12 elicits potent effects on the tumor growth, angiogenesis, and immunity. The net effects of CXCL12 in human tumor appear not to be beneficial.
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CXCL12 AND THERAPEUTIC APPLICATIONS |
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On the other hand, thousands of patients worldwide have received treatment with angiogenesis inhibitors or antagonists. Bevacizumab, a monoclonal antibody against VEGF, is one of them (53). Although administration of bevacizumab results in increased patient survival with certain cancers (20, 50, 56, 145), the clinical efficacy needs significant improvement. CXCL12 and VEGF synergistically induce tumor vascularization (66). It is thus expected that combination of anti-VEGF and anti-CXCL12 may be more effective.
Notably, CXCR4 and CXCL12 are expressed on multiple immune cells, vascular endothelial cells as well as stem cells. It is possible that targeting CXCR4/CXCL12 pathway may yield unexpected clinical effects. Nonetheless, it is evident that CXCR4/CXCL12 pathway is actively implicated in tumor pathogenesis and plays a significant role in tumor immunopathogenesis. Therefore, manipulation of this pathway represents new strategy for cancer treatment (154). Of course, we need to bear in mind that although targeting CXCR4/CXCL12 is an attractive option in treating human tumors, it is highly likely that to attain effective, reliable, and consistent clinical efficacy, a complicated combinatorial therapeutic regimen may be warranted.
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FOOTNOTES |
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 J. Kwong, H. Kulbe, D. Wong, P. Chakravarty, and F. Balkwill An antagonist of the chemokine receptor CXCR4 induces mitotic catastrophe in ovarian cancer cells Mol. Cancer Ther., July 1, 2009; 8(7): 1893 - 1905. [Abstract] [Full Text] [PDF]
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EXPRESSION OF CXCL12 IN...