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Fig. 2. A: mRNA levels of SERCA (a) and MyHCI (b) isoforms. B: ratio of SERCA2 and SERCA1 mRNAs and levels of SERCA1 mRNA in denervated and innervated regenerating soleus. Values are means ± SE, n = 3. Significant (*P < 0.05), very significant (**P < 0.01), and highly significant changes (***P < 0.001) during regeneration are indicated. In A, significant (°P < 0.05) and very significant differences (°°P < 0.01), and in B, significant +P < 0.05) and highly significant differences (+++P < 0.001), between innervated and denervated regenerated samples are indicated. Innerv. reg., innervated regenerating muscle; denerv. reg., denervated regenerating muscle. Note that MseI hydrolizes SERCA2 and NcoI hydrolizes SERCA1 fragment amplified by ratio RT PCR. Inset: 1 representative gel of 3 repeated experiments. In B, the column representing SERCA2/SERCA1 ratio in day 7 denervated regenerated muscle is 102 times higher than the scale of the ordinate; this is indicated in the column.
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