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Supplemental Movies

Supplemental Video Clips for figs. 3, 5a, and 5b.

Files in this Data Supplement:

• Video 3 - Image is of 3 CD63-GFP⁺ RBL cells in culture where a neurite extends from the left had side of the image and is in contact with the lower pseudopodial extension from the central RBL cell and the upper pseudopodium of the RBL cell to the right of the picture. Upon activation of the neurite with 10 nM bradykinin significant membrane ruffling occurs at the pseudopodial-neurite contact point. In contrast pseudopodia that are not in direct contact with the neurite (i.e. the lower extension from the RBL cell to the right of the image) are less active and significant membrane ruffling is not apparent.
• Video 5a - These video clips are representative of granule movements in CD63-GFP⁺ RBL cells. Figure 5A shows an RBL cell in contact with a neurite that is not activated. The granules in this cell are largely inactive and any movement is in a random direction and of short duration.
• Video 5b - In contrast the clip illustrating the RBL cell in figure 5B, where the neurite contact site is to the far right of the cell, shows significant granule movement particularly on the right-hand side of the RBL cell. For this clip the viewer should not focus on the left of the cell, which is in fact brighter because of more granules, rather attention is to be paid to the right side of the cell where major granule migrations occur.

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