| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 Institute for Fundamental and Clinical Human Movement Sciences, Vrije Universiteit, 1081 BT Amsterdam, The Netherlands; 2 Department of Physiology and Pharmacology, Karolinska Institutet, SE 171 77 Stockholm, Sweden; and 3 Neuromuscular Biology Research Group, Manchester Metropolitan University, Alsager, ST 7 2 HL, United Kingdom
 |
ABSTRACT |
|---|
 TOP
 ABSTRACT
 INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES
|
|---|
A brief high-frequency burst of action potentials results in a sustained force increase in skeletal muscle. The present study investigates whether this force potentiation is the result of a sustained increase of the free myoplasmic [Ca2+] ([Ca2+]i). Single fibers from mouse flexor brevis muscles were stimulated with three impulses at 150 Hz (triplet) at the start of a 350-ms tetanus or in the middle of a 700-ms tetanus; the stimulation frequency of the rest of the tetanus ranged from 20 to 60 Hz. After the triplet, force was significantly (P < 0.05) increased between 17 and 20% when the triplet was given at the start of the tetanus and between 5 and 18% when the triplet was given in the middle (n = 7). However, during this potentiation, [Ca2+]i was not consistently increased. Hence, the increased force following a high-frequency burst is likely due to changes in the myofibrillar properties.
force potentiation; excitation contraction coupling; mechanical output; skeletal muscle; Ca2+
| |
INTRODUCTION |
|---|
|
TOP
ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES
|
|---|
IN VIVO DISCHARGE PATTERNS of single motor units often start with a burst of two to three impulses at a high frequency. Such high-frequency bursts have been reported in humans (see e.g., Ref. 14) as well as in animals (see e.g., Ref. 10). The high-frequency burst is then followed by a much lower frequency of discharge. Studies with a high-frequency burst have shown sustained increases in force during isometric contractions (5) and in power output during concentric contractions (1).
It has been speculated that a high-frequency burst results in a maintained increase of the free myoplasmic [Ca2+] ([Ca2+]i) and, hence, increased activation of the contractile machinery (18, 24). A higher stimulation frequency undoubtedly results in increased [Ca2+]i and consequently increased force production (6, 22), but it is not clear whether the prolonged force increase after a high-frequency burst is due to a sustained increase of [Ca2+]i. To determine whether [Ca2+]i remains elevated after a high-frequency burst and whether this sustained elevation can explain the increased force, we performed simultaneous measurements of [Ca2+]i and force in single intact fibers isolated from mouse flexor digitorum brevis muscles. The fibers were stimulated with a triplet of 150-Hz impulses, given either at the start or in the middle of 20- to 60-Hz tetani. [Ca2+]i and force in these tetani were compared with measurements in control tetani without the triplet. The results showed a sustained force increase after the high-frequency triplet, whereas [Ca2+]i rapidly fell to the control level.
| |
MATERIALS AND METHODS |
|---|
|
TOP
ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES
|
|---|
Male mice (NMRI strain, weight ~30 g) were killed by rapid neck disarticulation, and intact single fibers were dissected from the flexor digitorum brevis muscle of the hindlimb following a procedure described by Lännergren and Westerblad (12). The isolated fiber was mounted between an Akers 801 force transducer and an adjustable holder. Fiber length was adjusted to that giving maximum tetanic force. The fiber was stimulated by supramaximal current pulses (duration 0.5 ms) delivered via platinum plate electrodes lying parallel to the long axis of the fiber.
Solutions. Experiments were performed at room temperature (24° C), i.e., somewhat less than the in situ temperature of the superficial muscle used (30.5°C) (4). The fiber was superfused by a Tyrode solution of the following composition (mM): 121 NaCl, 5.0 KCl, 1.8 CaCl2, 0.5 MgCl2, 0.4 NaH2PO4, 24.0 NaHCO3, 0.1 EDTA, and 5.5 glucose. Fetal calf serum (0.2%) was added to the solution, which was bubbled with 5% CO2-95% O2, giving a pH of 7.4.
[Ca2+]i measurements.
[Ca2+]i was measured with the fluorescent
Ca2+ indicator indo 1 as described previously
(2). Briefly, indo 1 (Molecular Probes Europe, Leiden, The
Netherlands) was microinjected into the fiber. The fluorescence of indo
1 was measured with a system consisting of a xenon lamp, a
monochromator, and two photomultiplier tubes (PTI, Wedel, Germany). The
excitation light was set to 360 ± 5 nm, and the light emitted at
405 ± 5 and 495 ± 5 nm was measured. The ratio of the light
emitted at 405 nm to that at 495 nm (R) was converted to
[Ca2+]i using the following equation
(8)
![[Ca<SUP>2+</SUP>]<SUB>i</SUB> = <IT>K</IT><SUB>d</SUB> &bgr; (R − R<SUB>min</SUB>) (R<SUB>max</SUB> − R)<SUP>−1</SUP>](/content/vol283/issue1/fulltext/C42/img001.gif)
|
is the ratio of the 495-nm signals at very low
and saturating [Ca2+]i, Rmin is
the ratio at very low [Ca2+]i, and
Rmax is the ratio at saturating
[Ca2+]i. Fluorescence and force signals were
sampled online and stored on a desktop computer system for subsequent
data analysis.
The kinetics of indo 1 are too slow to accurately follow rapid
transients of [Ca2+]i (25). This
problem can be reduced by kinetic correction of the indo 1 signal, and
such a correction has a large impact on [Ca2+]i measurements performed at the onset
of tetanic contractions. However, the effect of kinetic correction is
limited during the tetanic plateau (23). In the present
study, we compare [Ca2+]i measurements
obtained during the tetanic plateau. This comparison will be little
affected by the slow kinetics of indo 1, and, hence, no kinetic
correction of the indo 1 signal has been performed.
Experimental procedures. To study the effects of a high-frequency burst on [Ca2+]i and force, the isolated fiber was stimulated with three impulses at 150 Hz at the start of a 350-ms tetanus or in the middle of a 700-ms tetanus. The stimulation frequency during the rest of the tetanus was 20, 30, 40, or 60 Hz. Changes induced by the high-frequency triplet were assessed by measuring [Ca2+]i and force in tetani with triplet and in bracketing tetani produced at the same stimulation frequency but without the triplet. The order of the stimulation frequencies at which the effects of the triplet were studied was randomized. There was a resting period of 1 min between the individual contractions.
[Ca2+]i and force (in kPa) were measured as the mean between each stimulation pulse. Because this study focuses on sustained changes after the high-frequency triplet, force and [Ca2+]i were not compared in the time frame that included the triplet. Change in force and Ca2+ were calculated by subtracting data within the same time frame of a tetanic contraction with a triplet from the mean of bracketing contractions without a triplet. Change in force (
force) is expressed as a
percentage of the maximal tetanic force during a contraction at 120 Hz.
For each fiber, a force-[Ca2+]i curve was
constructed from measurements of force and
[Ca2+]i in tetani without a high-frequency
triplet produced at 20 to 120 Hz as described previously
(2).
To investigate the possible role of stretching of passive elements as a
mechanism of the force potentiation after a high-frequency triplet,
five fibers were stretched and the relative increase of force was
compared at the different lengths. In these experiments, the basal
stimulation frequency was 30 Hz while [Ca2+]i
and force were measured in the first time frame following the triplet.
At each fiber length, the force increase is expressed relative to the
maximum active force without a triplet. The relative force increase at
optimum length was set to 100% in each fiber. Fluorescence signals
were measured with a fixed field of view. This means that the fiber
volume within the field of view was reduced as the fiber was stretched,
and, hence, the fluorescence signals became smaller, which resulted in
more noise in the fluorescence ratio signals. Therefore, at long fiber
lengths, fluorescence ratios were filtered at 10 Hz, which reduced the
peak of [Ca2+]i transients and made them
broader (see Fig. 6), whereas the mean
[Ca2+]i was not affected. Thus a direct
quantitative comparison between [Ca2+]i
transients at normal and long length cannot be performed.
However, our main objective was to determine whether there was a
prolonged increase of [Ca2+]i after the
high-frequency triplet, and this would not be affected in
[Ca2+]i measurements at long length.
Statistics. Data are presented as means ± SE. Paired t-tests were used to establish significant differences between [Ca2+]i and force in tetani with and without a high-frequency triplet. The significance level was set to 0.05.
| |
RESULTS |
|---|
|
TOP
ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES
|
|---|
High-frequency triplet at the start of a contraction.
Figure 1 shows typical
[Ca2+]i and force records from one fiber
stimulated at 20 Hz with and without the high-frequency
triplet. The triplet resulted in a marked increase of both
[Ca2+]i and force. However, while force
remained elevated for about 200 ms after the triplet,
[Ca2+]i rapidly declined to the level
obtained without the triplet. Similar results were obtained at the
other frequencies tested, and results from all fibers are summarized in
Fig. 2. The change in Ca2+
showed no consistent increase after the triplet. On the other hand,
force was significantly (P < 0.05) increased by
16.6 ± 2.8% at 20 Hz, 16.9 ± 1.1% at 30 Hz, 17.8 ± 2.0% at 40 Hz, and 19.9 ± 2.5% at 60 Hz (n = 7)
in the first time frame after the triplet. Thereafter, force
decreased exponentially with an average rate constant of ~20
s
1.
|
|
0.18 ± 0.05 µM
(n = 7), calculated by assuming a simple parallel shift
of the force-[Ca2+]i curve.
|
High-frequency triplet in the middle of a tetanus.
Figure 4 shows representative
[Ca2+]i and force records from one fiber
stimulated for 700 ms at 20 Hz with and without a high-frequency triplet in the middle of the tetanus. The pattern is qualitatively similar to that observed when the triplet was given at the start of the
contraction: the increase of [Ca2+]i induced
by the triplet rapidly disappeared, whereas there was a sustained
increase in force. Mean data from all fibers (n = 7)
studied with a high-frequency triplet in the middle of the tetanus are
shown in Fig. 5. Similar to the situation
when a triplet was given at the start of tetani, there was no
consistent increase in [Ca2+]i after the
triplet. force in the first time frame after the triplet was
13.2 ± 2.6% at 20 Hz, 18.2 ± 3.4% at 30 Hz, 9.4 ± 1.9% at 40 Hz, and 4.5 ± 1.5% at 60 Hz [i.e., there was a
significant increase in force at all frequencies (P < 0.05)]. Fitting the decay of force to a single exponential function
in 20- and 30-Hz tetani (where the force increase was large enough to
give a reliable measure) gave a rate constant of ~20
s1. Compared with the force observed when the triplet
was given at the start of a contraction, force in the first
time frame was markedly smaller when the triplet was given in the
middle of tetani at 40 and 60 Hz. This can be explained by tetanic
force before the triplet starting to approach maximum force, which sets the limit on how much force can increase. For instance, in control tetani produced to obtain force-[Ca2+]i
curves (see Fig. 3), the force in 60-Hz tetani was 87.7 ± 1.9% of the force in 120-Hz tetani (i.e., maximum force). Therefore, the
maximum force potentiation would be limited to about 12%.
|
|
Effects of high-frequency triplets at long fiber lengths.
The effect of a high-frequency triplet at the beginning of a 30-Hz
tetanus was studied at optimal and increased length. Passive structures
in the fibers were stretched at the increased length, resulting in a
resting force of 50-200% of the maximum tetanic force at optimal
length. As a result, the filament overlap within sarcomeres was
reduced, resulting in a reduction of the active force in 30-Hz tetani
to <20% of that at optimal length (Fig. 6). There was a substantial force
potentiation after the high-frequency triplet also in stretched fibers,
although the relative force increase was somewhat smaller than at
optimal length (77.1 ± 3.5%; n = 5). At long
lengths, the high-frequency triplet resulted in an immediate increase
of [Ca2+]i, but in the first time frame after
the triplet, [Ca2+]i was not higher than in
bracketing tetani without triplets. Thus a high-frequency triplet,
while augmenting force production, did not result in a sustained
increase of [Ca2+]i either at optimal or
increased fiber length.
|
| |
DISCUSSION |
|---|
|
TOP
ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES
|
|---|
The main finding of the present study is that a high-frequency burst of impulses during a contraction at lower frequency results in a very brief increase in [Ca2+]i, which is followed by a prolonged force elevation. Thus a short period of high [Ca2+]i results in a prolonged increase in the activation of the contractile machinery, and possible mechanisms for this will be discussed in the following.
The present results show a leftward shift of the force-[Ca2+]i relationship after a high-frequency triplet (see Fig. 3). A similar shift in the force-[Ca2+]i relationship has been associated with increased myosin light chain phosphorylation (15, 20), which is thought to underlie other types of force potentiation such as posttetanic potentiation (16, 19, 21). Therefore, it may be speculated that a high-frequency triplet results in an increased level of myosin light chain phosphorylation. However, it is known that a relatively long tetanus is needed to induce a marked posttetanic potentiation (see e.g., Refs. 13, 21). Moreover, a study by Moore and Stull (16) on rat gastrocnemius muscle showed that dephosphorylation of the myosin light chain takes ~2 min, which is much longer than the duration of the effect of high-frequency triplets in the present experiments. Thus, considering the short duration of the present high-frequency triplets (~13 ms) and the short-lasting force potentiation, it seems very unlikely that an increased level of myosin light chain phosphorylation can account for the present force elevation after the triplet.
Another possible explanation for the present force potentiation after a triplet could be an enhanced force transmission due to stretching of passive elements of the muscle fiber. Parmiggiani and Stein (17) showed that two stimuli separated by a short interval produced three times the force of a single twitch in cat muscles (nonlinear summation of force). Because the increase in force was larger than the increase in muscle stiffness, which was assumed to be a measure for actin myosin bonds, Parmiggianni and Stein concluded that improved transmission of internal force was the only means for producing a net facilitation of force production. To study whether such a mechanism could also account for the sustained force potentiation following the triplet, we performed experiments at increased fiber length. Passive elements would be stretched already at rest at long lengths, and the effect of a short period of increased active force production would be small. Therefore, the force potentiation after a high-frequency triplet should be severely reduced at long lengths if the potentiation was due to stretching of passive elements. The present results show a slightly smaller triplet-induced relative force increase (~80%) in stretched fibers compared with fibers studied at optimal length. Thus improved force transmission through stretching of passive elements may account for ~20% of the present triplet-induced force potentiation, and the greater part of the potentiation must be due to some other mechanism.
The force-[Ca2+]i relationship was shifted to the left after the high-frequency triplet (see Fig. 3), i.e., there was an apparent increase of myofibrillar Ca2+ sensitivity. The interaction between Ca2+ activation of the actin filament and cross-bridge force production is rather complex (for a recent review, see Ref. 7). It is possible that the high-frequency triplet affects this interaction. For instance, Ca2+ binding to the regulatory sites of troponin C may be facilitated by attached, force-producing cross bridges (11). Thus the transient increase of [Ca2+]i during the triplet will result in an increased number of active cross bridges. This might then increase the Ca2+ affinity of regulatory sites on troponin C, and the activation of the actin filament would remain high despite declining tetanic [Ca2+]i. However, while important in cardiac myocytes, this mechanism appears to be of little importance in skeletal muscle fibers (7). Moreover, the present results showed the same rapid decline of [Ca2+]i to the control level after the triplet both at optimal and long length, and, hence, there were no signs of different Ca2+ buffering in the two situations. A mechanism that encompasses the present results is that the high-frequency triplet affects the actin-myosin interaction via direct effects of attached cross bridges on actin filament activation. Recent models of cross-bridge activation in skeletal muscle fibers suggest that Ca2+ binding to troponin C makes it possible for myosin heads to move tropomyosin from the sites where cross bridges bind. Once a cross bridge has moved tropomyosin, the binding of additional cross bridges to neighboring sites will be facilitated (7). This mechanism implies that there will be a delay in the force decline when [Ca2+]i is reduced because new force-bearing cross bridges can be formed close to already attached cross bridges despite lowered [Ca2+]i. In line with this model, the decrease of force markedly lags behind the decline of [Ca2+]i during relaxation of contractions (22).
The elevated force after the high-frequency triplet declined with a
rate constant of about 20 s1, which would be similar to
the rate of cross-bridge cycling under isometric conditions in
fast-twitch mammalian muscle fibers (~5 s1 in rabbit
psoas muscle fibers studied at 12°C) (9). Interestingly, the increased force after a burst of high-frequency stimulation is
maintained for a markedly longer period in slow-twitch skeletal muscle
(see e.g., Refs. 3 and 5), where the rate of cross-bridge cycling is lower than in fast-twitch skeletal muscle.
In conclusion, this study has shown that force potentiation after a high-frequency triplet is not the result of a sustained high [Ca2+]i. Rather, we suggest that the force potentiation is caused by an apparent increase in the myofibrillar Ca2+ sensitivity due to the facilitated formation of additional force-bearing cross bridges in the vicinity of already attached cross bridges.
| |
ACKNOWLEDGEMENTS |
|---|
This work was supported by grants from the Swedish Medical Research Council (Project 10842), the Swedish National Center for Sports Research, funds at the Karolinska Institutet, and the Netherlands Organization for Scientific Research.
| |
FOOTNOTES |
|---|
Address for reprint requests and other correspondence: F. Abbate, Dept. of Physiology and Pharmacology, Karolinska Institutet, SE 171 77 Stockholm, Sweden (E-mail: Fabio.Abbate{at}fyfa.ki.se).
The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
10.1152/ajpcell.00416.2001
Received 27 August 2001; accepted in final form 29 January 2002.
| |
REFERENCES |
|---|
|
TOP
ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES
|
|---|
1.
Abbate, F,
Sargeant AJ,
Verdijk PWL,
and
de Haan A.
The effects of high-frequency initial pulses and posttetanic potentiation on the power output of skeletal muscle.
J Appl Physiol
88:
35-40,
2000
2.
Andrade, FH,
Reid MB,
Allen DG,
and
Westerblad H.
Effect of hydrogen peroxide and dithiothreitol on contractile function of single skeletal muscle fibres from the mouse.
J Physiol
509:
565-575,
1998
3.
Binder-Macleod, SA,
and
Barrish WJ.
Force response of rat soleus muscle to variable frequency train stimulation.
J Neurophysiol
68:
1068-1078,
1992
4.
Bruton, JD,
Lännergren J,
and
Westerblad H.
Effects of CO2-induced acidification on the fatigue resistance of single mouse muscle fibers at 28°C.
J Appl Physiol
85:
478-483,
1998
5.
Burke, RE,
Rudomin P,
and
Zajac FE, III.
Catch property in single mammalian motor units.
Science
168:
122-124,
1970
6.
Blinks, JR,
Rüdel R,
and
Taylor SR.
Calcium transients in isolated amphibian skeletal muscle fibre: detection with aequorin.
J Physiol
277:
291-323,
1978
7.
Gordon, AM,
Homsher E,
and
Regnier M.
Regulation of contraction in striated muscle.
Physiol Rev
80:
853-924,
2000
8.
Grynkiewicz, G,
Poenie M,
and
Tsien RY.
A new generation of Ca2+ indicators with greatly improved fluorescence properties.
J Biol Chem
260:
3440-3450,
1985
9.
He, ZH,
Chillingworth RK,
Brune M,
Corrie JET,
Webb MR,
and
Ferenzi MA.
The efficiency of contraction in rabbit skeletal muscle fibres, determined from the rate of release of inorganic phosphate.
J Physiol
517:
839-854,
1999
10.
Hennig, R,
and
Lømo T.
Firing patterns of motor units in normal rats.
Nature
314:
164-166,
1985[Medline].
11.
Hofmann, PA,
and
Fuchs F.
Effect of length and cross-bridge attachment on Ca2+ binding to cardiac troponin C.
Am J Physiol Cell Physiol
253:
C90-C96,
1987
12.
Lännergren, J,
and
Westerblad H.
The temperature dependence of isometric contractions of single, intact fibres dissected from a mouse foot muscle.
J Physiol
390:
285-293,
1987
13.
Manning, DR,
and
Stull JT.
Myosin light chain phosphorylation-dephosphorylation in mammalian skeletal muscle.
Am J Physiol Cell Physiol
242:
C234-C241,
1982
14.
Marsden, CD,
Meadows JC,
and
Merton P
A. Isolated single motor units in human muscle and their rate of discharge during maximal voluntary effort.
J Physiol
217:
12-13,
1971.
15.
Metger, JM,
Greaser ML,
and
Moss RL.
Variation in cross-bridge attachment rate and tension with phosphorylation of myosin in mammalian skinned skeletal muscle fibers. Implications for twitch potentiation in intact muscle.
J Gen Physiol
93:
855-883,
1989
16.
Moore, RL,
and
Stull JT.
Myosin light chain phosphorylation in fast and slow skeletal muscles in situ.
Am J Physiol Cell Physiol
247:
C462-C471,
1984
17.
Parmiggiani, F,
and
Stein RB.
Nonlinear summation of contractions in cat muscles. II. Later facilitation and stiffness changes.
J Gen Physiol
78:
295-311,
1981
18.
Stevens, ED.
The pattern of stimulation influences the amount of oscillatory work done by frog muscle.
J Physiol
494:
279-285,
1996
19.
Sweeney, HL,
and
Stull JT.
Phosphorylation of myosin in permeabilized mammalian cardiac and skeletal muscle cells.
Am J Physiol Cell Physiol
250:
C657-C660,
1986
20.
Sweeney, HL,
and
Stull JT.
Alteration of cross-bridge kinetics by myosin light chain phosphorylation in rabbit skeletal muscle: implications for regulation of actin-myosin interaction.
Proc Natl Sci USA
87:
414-418,
1990
21.
Tubman, LA,
Rassier DE,
and
MacIntosh BR.
Attenuation of myosin light chain phosphorylation and post tetanic potentiation in atrophied skeletal muscle.
Pflügers Arch
434:
848-851,
1997[Web of Science][Medline].
22.
Westerblad, H,
and
Allen DG.
The influence of intracellular pH on contraction, relaxation and [Ca2+]i in intact single fibres from mouse muscle.
J Physiol
466:
611-628,
1993
23.
Westerblad, H,
and
Allen DG.
Mechanisms underlying changes of tetanic [Ca2+]i and force in skeletal muscle.
Acta Physiol Scand
156:
407-416,
1996[Web of Science][Medline].
24.
Zajac, FE,
and
Young JL.
Properties of stimulus trains producing maximum tension-time area per pulse from single motor units in medial gastrocnemius muscle of the cat.
J Neurophysiol
43:
1206-1220,
1980
25.
Zhao, M,
Hollingworth S,
and
Baylor SM.
Properties of tri- and tetracarboxylate Ca2+ indicators in frog skeletal muscle fibers.
Biophys J
70:
896-916,
1996[Web of Science][Medline].
This article has been cited by other articles:
|
|
 |
|
  N. Place, T. Yamada, J. D. Bruton, and H. Westerblad Interpolated twitches in fatiguing single mouse muscle fibres: implications for the assessment of central fatigue J. Physiol., June 1, 2008; 586(11): 2799 - 2805. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. G. Allen, G. D. Lamb, and H. Westerblad Skeletal Muscle Fatigue: Cellular Mechanisms Physiol Rev, January 1, 2008; 88(1): 287 - 332. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. K. Hager-Ross, C. S. Klein, and C. K. Thomas Twitch and Tetanic Properties of Human Thenar Motor Units Paralyzed by Chronic Spinal Cord Injury J Neurophysiol, July 1, 2006; 96(1): 165 - 174. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. J. Aughey, C. J. Gore, A. G. Hahn, A. P. Garnham, S. A. Clark, A. C. Petersen, A. D. Roberts, and M. J. McKenna Chronic intermittent hypoxia and incremental cycling exercise independently depress muscle in vitro maximal Na+-K+-ATPase activity in well-trained athletes J Appl Physiol, January 1, 2005; 98(1): 186 - 192. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S M Baylor and S Hollingworth Sarcoplasmic reticulum calcium release compared in slow-twitch and fast-twitch fibres of mouse muscle J. Physiol., August 15, 2003; 551(1): 125 - 138. [Abstract] [Full Text] [PDF]
|
|
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
) and
[Ca2+]i (
) between tetani
with and without an initial high-frequency triplet. Stimulation
frequency was 20 Hz (A), 30 Hz (B), 40 Hz
(C), and 60 Hz (D). Time axes start at the onset
of contractions. Dashed line indicates no difference. Mean 
) after the triplet, force was
markedly increased, whereas [Ca2+]i showed
little change. Force declined in subsequent time frames, and in the
fourth and fifth time frame (250-300 ms; smallest
