Supplemental Video

Rac, but not Rho signaling, protects epithelial adherens junction assembly during ATP-depletion.

Shobha Gopalakrishnan, K. W. Dunn and James A. Marrs.

Rendered volumes of images in Figure 1A. MDCK cells were stained for immunofluorescence using antibodies specific for E-cadherin or β-catenin. FITC-conjugated secondary antibodies were used to visualize E-cadherin or β-catenin distributions. Stacks of approximately 100 x-y image planes were collected through the volume of the cell monolayer using a two-photon microscope. Movie files were generated using the Voxx software, converted to a video clip using Quicktime (Apple Computer Inc.), and compressed using MPEG encoder (TMPGEnc). Animations of image volumes were rendered using Voxx. Click on the still images to view the video.

E-cadherin localization in contol MDCK cells (three-dimensional rendering of 137 image planes).

E-cadherin localization in MDCK cells ATP-depleted for 30 minutes (three-dimensional rendering of 97 image planes).

E-cadherin localization in MDCK cells ATP-depleted for 60 minutes (three-dimensional rendering of 111 image planes).

β-catenin localization in control MDCK cells (three-dimensional rendering of 105 image planes).

β-catenin localization in MDCK cells ATP-depleted for 30 minutes (three-dimensional rendering of 192 image planes).

β-catenin localization in MDCK cells ATP-depleted for 60 minutes (three-dimensional rendering of 151 image planes).

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