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Am J Physiol Cell Physiol 282: C451-C460, 2002; doi:10.1152/ajpcell.00333.2001
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Alignment of the Sequences of the 220-kDa MLCKs

The deduced amino acid sequence of the mouse 220-kDa myosin light chain kinase (MLCK) is shown aligned to the sequences reported for the human endothelial MLCK and the avian nonmuscle MLCK (1, 2). The consensus sequence is shown below the alignment; lower case letters indicate that two sequences are identical. Arrows and boldface methionine residues identify the predicted initiation sites for 130-kDa MLCK and telokin.

  1. Garcia JG, Verin AD, and Schaphorst KL. Regulation of thrombin-mediated endothelial cell contraction and permeability. Semin Thromb Hemost 22: 309-315, 1996.
  2. Watterson DM, Collinge M, Lukas TJ, Van Eldik LJ, Birukov KG, Stepanova OV, and Shirinsky VP. Multiple gene products are produced from a novel protein kinase transcription region. FEBS Lett 373: 217-220, 1995.

 

            1                                                   50
     HUMAN                                                  MGDVKL
     MOUSE  MNFNFTLPFC LRILESCRGE LVSLPGFPRE PLCESMSGQL PGKTMGDVKL
     CHICK                                                  MGDVKL
 Consensus                                                  MGDVKL

            51                                                 100
     HUMAN  VASSHISKTS LSVDPSRVDS MPLTEAPAFI LPPRNLCIKE GATAKFEGRV
     MOUSE  FASSHMSKTS HSVDPSKVSS MPLTEAPAFI LPPRNLCVKE GATAKFEGRV
     CHICK  VTSTRVSKTS LTLSPS---- VP-AEAPAFT LPPRNIRVQL GATARFEGKV
 Consensus  vaSsh SKTS lsvdPS.v s mPltEAPAFi LPPRNlc.ke GATAkFEGrV

            101                                                150
     HUMAN  RGYPEPQVTW HRNGQPITSG GRFLLDCGIR GTFSLVIHAV HEEDRGKYTC
     MOUSE  RGYPEPQVTW HRKGQAITNG GRFLLDCGVR GTFSLVIHTV REEDKGKYTC
     CHICK  RGYPEPQITW YRNGHPLPEG DHYVVDHSIR GIFSLVIKGV QEGDSGKYTC
 Consensus  RGYPEPQ.TW hRnGqpit G gr.llDcg.R GtFSLVIh V  EeD.GKYTC

            151                                                200
     HUMAN  EATNGSGARQ VTVELTVEGS FAKQLGQPVV SKTLGDRFSA SAVETRPSIW
     MOUSE  EASNGSGARQ VTVELTVEGN SMKKRDQPVL SKASG----- FPGETRPSIW
     CHICK  EAANDGGVRQ VTVELTVEGN SLKKYSLPSS AKTPGGRLSV PPVEHRPSIW
 Consensus  EA NgsGaRQ VTVELTVEGn s Kk  qPv  sKt G r s   pvEtRPSIW

            201                                                250
     HUMAN  GECPPKFATK LGRVVVKEGQ MGRFSCKITG RPQPQVTWLK GNVPLQPSAR
     MOUSE  GECPPKFATK LGRAVVKEGQ MGRFSCKITG RPPPQVTWLK GNVPLQPSAR
     CHICK  GESPPKFATK PNRVVVREGQ TGRFSCKITG RPQPQVTWTK GDIHLQQNER
 Consensus  GEcPPKFATK lgRvVVkEGQ mGRFSCKITG RPqPQVTWlK G..pLQpsaR

            251                                                300
     HUMAN  VSVSEKNGMQ VLEIHGVNQD DVGVYTCLVV NGSGKASMSA ELSIQGLDSA
     MOUSE  VSMSEKNGMQ ILEIRGVTRD DLGVYTCMVV NGSGKASMSA ELSIPGLDNA
     CHICK  FNMFEKTGIQ YLEIQNVQLA DAGIYTCTVV NSAGKASVSA ELTVQGPDKT
 Consensus  vsmsEKnGmQ .LEI gV  d D G.YTC VV NgsGKASmSA Els.qGlD a

            301                                                350
     HUMAN  N--------- -RSFVRETKA -TNSDVRKEV TNVISKESKL DSLEAAAKSK
     MOUSE  S--------- -RLAVRGTKA -PSPDIRKEV TNGVSKDP-- ---ETVAESK
     CHICK  DTHAQPLCMP PKPTTLATKA IENSDFKQAT SNGIAKELKS TSTELMVETK
 Consensus              r  vr TKA   nsD rkev tNg.sK. k   s E  aesK

            351                                                400
     HUMAN  N-CSSPQRGG SPPWAANSQP QPPRESKLES CKDSPRTAPQ TPVLQKTSSS
     MOUSE  N-CPSPQRSG SSARATNSHL KSPQEPKPKL CEDAPRKVPQ SSILQKSTST
     CHICK  DRLSAKKETF YTSREAKDGK QGQNQEANAV PLQESRGTKG PQVLQKTSST
 Consensus  . csspqr g s  raans   q p . k    c . pR  pq   .LQKtsSt

            401                                                450
     HUMAN  ITLQAARVQP EPRAPGLGVL SPSGEERKRP APPRPATFPT RQPGLGSQ--
     MOUSE  ITLQALKVQP EARVPAIGSF SP-GEDRKSL AAPQQATLPT RQSSLGGS--
     CHICK  ITLQAVKAQP EPKAEPQTTF IRQAEDRKRT VQPLMTTTTQ ENPSLTGQVS
 Consensus  ITLQA kvQP Eprap  g f sp gE.RKr  a P  aT pt r.psLggq  

            451                                                500
     HUMAN  ----DVVSKA ANRRIPMEGQ RDSAF--PKF ESKPQSQEVK ENQTVKFRCE
     MOUSE  -----VGNKF VTGNIPRESQ RESTF--PRF ESQPQSQEVT EGQTVKFICE
     CHICK  PRSRETENRA GVRKSVKEEK REPLGIPPQF ESRPQSLEAS EGQEIKFKSK
 Consensus       v nka   r ip E q R.s f  P.F ES PQSqEv  EgQt.KF ce

            501                                                550
     HUMAN  VSGIPKPEVA WFLEGTPVRR QEGSIEVYED AGSHYLCLLK ARTRDSGTYS
     MOUSE  VSGIPKPDVG WFLEGIPVRR REGITEVYED GVSHHLCLLR ARTRDSGRYS
     CHICK  VSGKPKPDVE WFKEGVPIKT GEG-IQIYEE DGTHCLWLKK ACLGDSGSYS
 Consensus  VSGiPKP.V  WFlEG P.rr  EG i..YE.  gsH LcLlk ArtrDSG YS

            551                                                600
     HUMAN  CTASNAQGQV SCSWTLQVER LAVMEVAPSF SSVLKDCAVI EGQDFVLQCS
     MOUSE  CTATNSLAQV SCSWSLLVDR PNLAQTAPSF SSVLKDSVVI EGQDFVLRCS
     CHICK  CAAFNPRGQT STSWLLTVKR PKVEEVAPCF SSVLKGCTVS EGQDFVLQCY
 Consensus  CtA N  gQv ScSW L V.R p v .vAPsF SSVLKdc Vi EGQDFVLqCs

            601                                                650
     HUMAN  VRGTPVPRIT WLLNGQPIQY ARSTCEAGVA ELHIQDALPE DHGTYTCLAE
     MOUSE  VQGTPAPRVT WLLNGQPIQF AHSICEAGVA ELHIQDALPE DRGTYTCLAE
     CHICK  VGGVPVPEIT WLLNEQPIQY AHSTFEAGVA KLTVQDALPE DDGIYTCLAE
 Consensus  V GtPvPr.T WLLNgQPIQ. AhStcEAGVA eLh.QDALPE D GtYTCLAE

            651                                                700
     HUMAN  NALGQVSCSA WVTVHEKKSS RKSEYLLPVA PSKPTAPIFL QGLSDLKVMD
     MOUSE  NAMGQVSCSA TVTVQEKKGE GEREHRLSPA RSKPIAPIFL QGLSDLKVMD
     CHICK  NNAGRASCSA QVTVKEKKSS KKAEGTQAAK LNKTFAPIFL KGLTDLKVMD
 Consensus  Na GqvSCSA  VTV EKKss  k E  l  a  sKp APIFL qGLsDLKVMD

            701                                                750
     HUMAN  GSQVTMTVQV SGNPPPEVIW LHNGNEIQES EDFHFEQRGT QHSLWIQEVF
     MOUSE  GSQVTMTVQV SGNPPPEVIW LHDGNEIQES EDFHFEQKGG WTPLCIQEVF
     CHICK  GSQVIMTVEV SANPCPEIIW LHNGKEIQET EDFHFEKKGN EYSLYIQEVF
 Consensus  GSQVtMTV.V SgNPpPE.IW LH.GnEIQEs EDFHFEqkG    sL IQEVF

            751                                                800
     HUMAN  PEDTGTYTCE AWNSAGEVRT QAVLTVQEPH DGTQPWFISK PRSVTASLGQ
     MOUSE  PESTGTYTCE AWNSAGEVRT RAVLTVQEPH DGTQPWFISK PRSVTATLGQ
     CHICK  PEDTGKYTCE AWNELGETQT QATLTVQEPQ DGIQPWFISK PRSVTAAAGQ
 Consensus  PEdTGtYTCE AWNsaGEvrT qAvLTVQEPh DGtQPWFISK PRSVTA lGQ

            801                                                850
     HUMAN  SVLISCAIAG DPFPTVHWLR DGKALCKDTG HFEVLQNEDV FTLVLKKVQP
     MOUSE  SVLISCAIAG DPFPTVHWLR DGRASPKDSG HFELLQNEDV FTLVLKNVQP
     CHICK  NVLISCAIAG DPFPTVHWFK DGQEITPGTG -CEILQNEDI FTLILRNVQS
 Consensus  sVLISCAIAG DPFPTVHWlr DG.a  kdtG hfE LQNED. FTL.LknVQp

            851                                                900
     HUMAN  WHAGQYEILL KNRVGECSCQ VSLMLQNSSA ---RALPRGR EPASCEDLCG
     MOUSE  WHAGQYEILL KNRVGECSCQ VSLMLHNSPS ---RAPPRGR EPASCEGLCG
     CHICK  RHAGQYEIQL RNQVGECSCQ VSLMLRESSA SRAEMLRDGR ESASSGERRD
 Consensus  wHAGQYEIlL kNrVGECSCQ VSLML .Ssa    ralprGR EpASce.lcg

            901                                                950
     HUMAN  GGVGADGGGS DRYGSLRPGW PARGQGWLEE EDGEDVRGVL KRRVETRQHT
     MOUSE  GGGVGAHGDG DRHGTLRPCW PARGQGWPEE EDGEDVRGLL KRRVETRLHT
     CHICK  GGNYGALTFG RTSGFKKSSS ETRAA----E EEQEDVRGVL KRRVETREHT
 Consensus  GG  ga g g dr G lrp w paRgqgw eE E.gEDVRGvL KRRVETR HT

            951                              130 kDa -->  1000
     HUMAN  EEAIRQQEVE QLDFRDLLGK KVSTKTLSED DLKEIPAEQM DFRANLQRQV
     MOUSE  EEAIRQQEVG QLDFRDLLGK KVSTKTVSED DLKDIPAEQM DFRANLQRQV
     CHICK  EESLRQQEAE QLDFRDILGK KVSTKSFSEE DLKEIPAEQM DFRANLQRQV
 Consensus  EEaiRQQEve QLDFRDlLGK KVSTKt SE. DLK.IPAEQM DFRANLQRQV

    1001                                              1050
     HUMAN  KPKTVSEEER KVHSPQQVDF RSVLAKKGTP KTPVPEKVPP PKPATPDFRS
     MOUSE  KPKTISEEER KVHSPQQVDF RSVLAKKGTP KTPVPEKAPP -KAATPDFRS
     CHICK  KPKTLSEEER KVHAPQQVDF RSVLAKKGTP KTPLPEKVPP PKPAVTDFRS
 Consensus  KPKT SEEER KVHsPQQVDF RSVLAKKGTP KTPvPEKvPP pKpAtpDFRS

            1051                                              1100
     HUMAN  VLGGKKKLPA ENGSSSAETL NAKAVESSKP LSNAQPSGPL KPVGNAKPAE
     MOUSE  VLGGKKKSPS ENGGNSAEVL NVKAGESPTP AGDGQAIGAL KPVGNAKPAE
     CHICK  VLGAKKKPPA ENGSASTPAP NARAGSEAQN ATPNSEAPAP KPVVKKEEKN
 Consensus  VLGgKKK Pa ENGs Sae l NakAges  p a   q  gal KPVgnakpa.

            1101                                              1150
     HUMAN  TLKPMGNAKP AETLKPMGNA KPDENLKSAS K--------- ----------
     MOUSE  TPKPIGNAKP TETLKPVANT KPAETLKPIA NAQPSGSLKP VTNAQPAEPQ
     CHICK  DRKCEHGCAV VDG------- ---------- ---------- ----------
 Consensus  t Kp gnakp  .tlkp  n  kp e lk                         

            1151                                              1200
     HUMAN  ---------- ------EELK KDVKNDVNCK RGHAGTTDNE KRSESQGTAP
     MOUSE  KPVGNAKSAE TSKPAGKEEV KEVKNDVNCK KGQVGRTGNE KRPESQGSAP
     CHICK  ---------- ---------- ----GIIGKK AENKPAASKP TPPPSKGTAP
 Consensus                    e   k.vknd.ncK  g  g t ne krpeSqGtAP

            1201                                              1250
     HUMAN  AFKQKLQDVH VAEGKKLLLQ CQVSSDPPAT IIWTLNGKTL KTTKFIILSQ
     MOUSE  VFKEKLQDVH VAEGEKLLLQ CQVISDPPAT VTWSLNGKTL KTTKFIVLAQ
     CHICK  SFTEKLQDAK VADGEKLVLQ CRISSDPPAS VSWTLDSKAI KSSKSIVISQ
 Consensus   Fk.KLQDvh VA.GeKLlLQ Cq.sSDPPAt . WtL.gKtl KttKfI.lsQ

            1251                                              1300
     HUMAN  EGSLCSVSIE KALLEDRGLY KCVAKNDAGQ AECSCQVTVD DAPASENTKA
     MOUSE  EGSTFLCLHR EALPEDRGLY KCVAKNSAGQ AECSCQVTVA DAQTSENTKA
     CHICK  EGTLCSLTIE KVMPEDGGEY KCIAENAAGK AECACKVLVE DTSSTKAAKP
 Consensus  EGslcs  ie ka.pEDrGlY KC.AkN AGq AECsCqVtV  Da  sentKa

            1301                                              1350
     HUMAN  PEMKSRRPKS SLPPVLGTES -DATVKKKPA PKTPPKAAMP PQIIQFPEDQ
     MOUSE  PEMKSRRPKS SLPPVLGTES -DATVKKKPA PKTPTKAAMP PQIIQFPEDQ
     CHICK  AEKKTKKPKT TLPPVLSTES SEATVKKKPA PKTPPKAATP PQITQFPEDR
 Consensus  pEmKsrrPKs sLPPVLgTES  .ATVKKKPA PKTPpKAAmP PQIiQFPEDq

            1351                                              1400
     HUMAN  KVRAGESVEL FGKVTGTQPI TCTWMKFRKQ IQDSEHIKVE NSENGSKLTI
     MOUSE  KVRAGEPVEL FGKVAGTQPT TCKWMKFRKQ IQESEHIKVE NGESGSKLTI
     CHICK  KVRAGESVEL FAKVVGTAPI TCTWMKFRKQ IQENEYIKIE NAENSSKLTI
 Consensus  KVRAGEsVEL FgKV GTqPi TCtWMKFRKQ IQ.sEhIK.E N EngSKLTI

            1401                                              1450
     HUMAN  LAARQEHCGC YTLLVENKLG SRQAQVNLTV VDKPDPPAGT PCASDIRSSS
     MOUSE  LAARQEHCGC YTLVVENKLG SRQAQVNLTV VDKPDPPAGT PCASDIRSSS
     CHICK  SSTKQEHCGC YTLVVENKLG SRQAQVNLTV VDKPDPPAGT PCASDIRSSS
 Consensus  laarQEHCGC YTLvVENKLG SRQAQVNLTV VDKPDPPAGT PCASDIRSSS

            1451                                              1500
     HUMAN  LTLSWYGSSY DGGSAVQSYS IEIWDSANKT WKELATCRST SFNVQDLLPD
     MOUSE  LTLSWYGSSY DGGSAVQSYN VEIWDTEDKV WKELATCRSH SFNVQDLLPD
     CHICK  LTLSWYGSSY DGGSAVQSYT VEIWNSVDNK WTDLTTCRST SFNVQDLQAD
 Consensus  LTLSWYGSSY DGGSAVQSY  .EIW.s .k  Wk.LaTCRSt SFNVQDLlpD

            1501                                              1550
     HUMAN  HEYKFRVRAI NVYGTSEPSQ ESELTTVGEK PEEP--KMKW RCQTDDEKEP
     MOUSE  REYKFRVRAV NVYGTSEPSQ ESELTAVGEK PEEP--KDEV EVSDDDEKEP
     CHICK  REYKFRVRAA NVYGISEPSQ ESEVVKVGEK QEEELKEEEA ELSDDEGKET
 Consensus  rEYKFRVRA  NVYGtSEPSQ ESElt VGEK pEEp  k e  e sdD.eKEp

            1551                                              1600
     HUMAN  EVDYRTVTIN TEQKVSDFYD IEERLGSGKF GQVFRLVEKK TRKVWAGKFF
     MOUSE  EVDYRTVTVN TEQKVSDVYD IEERLGSGKF GQVFRLVEKK TGKIWAGKFF
     CHICK  EVNYRTVTIN TEQKVSDVYN IEERLGSGKF GQVFRLVEKK TGKVWAGKFF
 Consensus  EV.YRTVT.N TEQKVSDvY. IEERLGSGKF GQVFRLVEKK TgK.WAGKFF

            1601                                              1650
     HUMAN  KAYSAKEKEN IRQEISIMNC LHHPKLVQCV DAFEEKANIV MVLEIVSGGE
     MOUSE  KAYSAKEKDN IRQEISIMNC LHHPKLVQCV DAFEEKANIV MVLEIVSGGE
     CHICK  KAYSAKEKEN IRDEISIMNC LHHPKLVQCV DAFEEKANIV MVLEMVSGGE
 Consensus  KAYSAKEK.N IR.EISIMNC LHHPKLVQCV DAFEEKANIV MVLEiVSGGE

            1651                                              1700
     HUMAN  LFERIIDEDF ELTERECIKY MRQISEGVEY IHKQGIVHLD LKPENIMCVN
     MOUSE  LFERIIDEDF ELTERECIKY MRQISEGVEY IHKQGIVHLD LKPENIMCVN
     CHICK  LFERIIDEDF ELTERECIKY MRQISEGVEY IHKQGIVHLD LKPENIMCVN
 Consensus  LFERIIDEDF ELTERECIKY MRQISEGVEY IHKQGIVHLD LKPENIMCVN

            1701                                              1750
     HUMAN  KTGTRIKLID FGLARRLENA GSLKVLFGTP EFVAPEVINY EPISYATDMW
     MOUSE  KTGTRIKLID FGLARRLENA GSLKVLFGTP EFVAPEVINY EPIRYATDMW
     CHICK  KTGTSIKLID FGLARRLESA GSLKVLFGTP EFVAPEVINY EPIGYETDMW
 Consensus  KTGTrIKLID FGLARRLEnA GSLKVLFGTP EFVAPEVINY EPI YaTDMW

            1751                                              1800
     HUMAN  SIGVICYILV SGLSPFMGDN DNETLANVTS ATWDFDDEAF DEISDDAKDF
     MOUSE  SIGVICYILV RGLSPFMGDN DNETLANVTS ATWDFDDEEF DEISDDAKDF
     CHICK  SIGVICYILV SGLSPFMGDN DNETLANVTS ATWDFDDEAF DEISDDAKDF
 Consensus  SIGVICYILV sGLSPFMGDN DNETLANVTS ATWDFDDEaF DEISDDAKDF

            1801                                              1850
     HUMAN  ISNLLKKDMK NRLDCTQCLQ HPWLMKDTKN MEAKKLSKDR MKKYMARRKW
     MOUSE  ISNLLKKDMK NRLDSTHGLQ HPWLMKDTKN MEAKKLSKDR MKKYMARRKW
     CHICK  ISNLLKKDMK SRLNCTQCLQ HPWLQKDTKN MEAKKLSKDR MKKYMARRKW
 Consensus  ISNLLKKDMK nRL.cTqcLQ HPWLmKDTKN MEAKKLSKDR MKKYMARRKW

            1851  Telokin -->                             1900
     HUMAN  QKTGNAVRAI GRLSSMAMIS GLSGRKSSTG SPTSPLNAEK LESED-VSQA
     MOUSE  QKTGNAVRAI GRLSSMAMIS GLSGRKSSTG SPTSPINAEK LESEDDVSQA
     CHICK  QKTGHAVRAI GRLSSMAMIS GMSGRKASGS SPTSPINADK VENED----A
 Consensus  QKTGnAVRAI GRLSSMAMIS GlSGRKsStg SPTSPiNA.K lEsED vsqA

            1901                                              1950
     HUMAN  FLEAVAEEKP HVKPYFSKTI RDLEVVEGSA ARFDCKIEGY PDPEVVWFKD
     MOUSE  FLEAVAEKKP HVKPYFSKTI RDLEVVEGSA ARFDCKIEGY PDPEVVWFKD
     CHICK  FLEEVAEEKP HVKPYFTKTI LDMEVVEGSA ARFDCKIEGY PDPEVMWYKD
 Consensus  FLEaVAEeKP HVKPYFsKTI rDlEVVEGSA ARFDCKIEGY PDPEVvW.KD

            1951                                              2000
     HUMAN  DQSIRESRHF QIDYDEDGNC SLIISDVCGD DDAKYTCKAV NSLGEATCTA
     MOUSE  DQSIRESRHF QIDYDEDGNC SLIISDVCGD DDAKYTCKAV NSLGEATCTA
     CHICK  DQPVKESRHF QIDYDEEGNC SLTISEVCGD DDAKYTCKAV NSLGEATCTA
 Consensus  DQs.rESRHF QIDYDE.GNC SliIS.VCGD DDAKYTCKAV NSLGEATCTA

            2001                    2026
     HUMAN  ELIVETM-EE GEGE--GEEE E     
     MOUSE  ELIVETM-EE GEGEEGGEEE EEEEE 
     CHICK  ELLVETMGKE GEGEGEGEED EEEEEE
 Consensus  ELiVETM eE GEGE  GEE. Eeeee




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