Vol. 275, Issue 3, Ca1-Ca1, September 1998
CORRIGENDA
Volume 269, September 1995 Volume 38, September 1995
Pages C641-C654: C. M. Fuller, M. S. Awayda, M. P. Arrate, A. L. Bradford, R. G. Morris, C. M. Canessa, B. C. Rossier, and D. J. Benos. "Cloning of a bovine renal
epithelial Na+ channel subunit." This
article contains two errors in the published sequence of the cDNA
bENaC clone presented in Fig. 1. A severe C compression
at nucleotide positions 1750 and 1760 resulted in a double frameshift
in the C-terminal portion of the sequence. Correction of the nucleotide
sequence causes the termination codon to fall at position 1951 (as
opposed to position 2092 as previously published), predicting a
translated polypeptide of 650 amino acids as opposed to 697 residues as
previously reported. This shortened protein has a calculated molecular
mass of 73.4 kDa, although it is observed to migrate with an
Mr of ~80,000 on 8%
SDS-PAGE. The overall homology of the nucleotide sequence with the rat
and human ENaC clones is slightly increased by this
sequence change to 80% and 84% identities, respectively. In the C
terminal region, the identities (similarities) for human/bovine are now
64% (74%) and 55% (68%) for bovine/rat. A revised nucleotide and
amino acid sequence is given in Fig. 1. The sites of the C insertion
are underlined and the altered amino acid sequence is given in bold. Based on the observation of a major difference in the C-terminus of
bovine ENaC (an extra 47 amino acids), we proposed that
bENaC was a novel isoform of the ENaC gene family. This
conclusion is no longer supported by the present findings. However,
this sequence revision does not affect the other data or conclusions of
this study. The amended sequence has been deposited with GenBank
(Accession # U14944). The authors apologize for any inconvenience
caused by this error. B. C. Rossier wishes to withdraw his name from the original paper.
Am J Physiol Cell Physiol 275(3):Ca1-Ca1
