Supplemental Videos

Two videos in Quicktime format, 1 figure in PDF format.

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• Figure S1 - Large cytoplasmic vesicles containing ssYFP represent enlargements of the ER. LLC-AQP2-ssYFP cells were (A) singly immunostained either ER/cis-Golgi marker anti-BiP alone or (B) doubly stained with anti-BiP and anti-YFP to highlight unfolded/non-fluorescent ssYFP (B). While native YFP fluorescence is more prominent in a perinuclear area devoid of BiP, intense co-localization between BiP and ssYFP is observed in large cytoplasmic vesicles that do not stain for endosomal or lysosomal markers. We therefore conclude that these must represent enlargements of the ER, and are unlikely to be exocytotic.
• Video 1 - Rotation of 3D reconstruction of LLC-AQP2-ssYFP cells, unstained, showing only native YFP fluorescence. ssYFP appears as a tubular and vesicular pattern, showing prominent staining in a Golgi-like perinuclear compartment and a low-intensity diffuse punctate staining throughout the cytoplasm presumed to be small exocytotic vesicles.
• Video 2 - Rotation of 3D reconstruction of LLC-AQP2-ssYFP cells loaded with Alexa-555-dextran (1.5 mg/ml, red) for 15 min. Native ssYFP fluorescence is shown in green. No significant co-localization between the endocytotic marker and ssYFP is observed.