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Supplemental Videos

Five supplemental .AVI files:

Files in this Data Supplement:

• Video 1 - MTX-induced loss of GFP from single BAECs. The time-lapse video of the experiment shown in Fig 5 was created from the captured images as described in Materials and Methods, with a time compression of 3.5 min (i.e., 7 images) per second. The phase and GFP fluorescence images, taken every 30 sec for 30 min, were merged into a single video with GFP fluorescence shown as green pseudocolor.
• Video 2 - Simultaneous measurement of MTX-induced GFP loss and EB uptake. The time-lapse video of the cell shown in the montage of Fig 6 was created from the captured images as described in Materials and Methods, with a time compression of 3.5 min (i.e., 7 images) per second. The phase, GFP and EB fluorescence images, taken every 30 sec for 40 min, were merged into a single video with GFP fluorescence shown as green pseudocolor and EB fluorescence shown as red pseudocolor.
• Video 3 - Simultaneous measurement of MTX-induced GFP loss and EB uptake. The time-lapse video of the experiment shown quantified in the graph of Fig 6 (entire field of view) was created from the captured images as described in Materials and Methods, with a time compression of 3.5 min (i.e., 7 images) per second. The phase, GFP and EB fluorescence images, taken every 30 sec for 40 min, were merged into a single video with GFP fluorescence shown as green pseudocolor and EB fluorescence shown as red pseudocolor.
• Video 4 - Effect of glycine removal on GFP loss and EB uptake. The time-lapse video of the experiment shown quantified in the graph shown in Fig 8 (bottom) was created from the captured images as described in Materials and Methods, with a time compression of 3.5 min (i.e., 7 images) per second. The phase, GFP and EB fluorescence images, taken every 30 sec for 80 min, were merged into a single video with GFP fluorescence shown as green pseudocolor and EB fluorescence shown as red pseudocolor.
• Video 5 - Free-floating membrane blebs following MTX challenge. The time-lapse video was created from the captured images as described in Materials and Methods, with a time compression of 3.5 min (i.e., 7 images) per second. The phase and PI fluorescence images, taken every 10 sec for 15 min, were merged into a single video with PI fluorescence shown as red pseudocolor. The experimental conditions were identical to those described in the legend to Fig 9 in the absence of glycine. During the course of this video, large free-floating membrane blebs can be seen moving into and across the field of view.

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