Supplemental Movies

Five movies in MPG format.

Files in this Data Supplement:

• Movie 1a - Time-lapse sequence of a 4-minute recording of Fluo-4 and MitoTracker fluorescence in a cortical neuron. Mitochondria are observed as punctate fluorescent structures, whereas cytosolic Ca2+ transients caused by spontaneous firing of action potentials can be observed as increases in the background fluorescence of the entire neuron. Mitochondrial movement is not affected by the Ca2+ transients.
• Movie 1b - Same recording as in 1A but with fluorescence represented with an inverse grayscale, so that mitochondria are dark, and calcium transients appear as dark flashes in the entire neuron.
• Movie 2a - Second example of time-lapse sequence of a 6 minute recording of Fluo-4 and MitoTracker fluorescence in a cortical neuron. Again, mitochondrial movement is not affected by transient increases in Ca2+i associated with action potential firing.
• Movie 2b - Same recording as in 2A but with inverse grayscale.
• Movie 4 - High magnification sequence showing Fluo-4 and MitoTracker fluorescence in a single dendrite of a cortical neuron as in Figure 4b. Time lapse sequence of a 90 second recording shows the same mitochondrian as the kymograph in 4a. The movement of the mitochondrian is unaffected by multiple spontaneous Ca2+ transients.