An ecdysone and tetracycline dual regulatory expression system for studies on Rac1 small GTPase-mediated signaling
Am J Physiol Cell Physiol
Lai et al. 10.1152/ajpcell.00064.2003.
Supplemental Figures
Full images are shown of parellelly processed and silver-stained gels from cell lysates as described in Fig. 5, C–F. HEK-293.Ec.3.+2-2 double regulated cells were cotransfected with pU-MRac1-V12 and pIND-IκB(ƒvDN).
Files in this Data Supplement:
- Supplemental Figure A
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Neither doxycycline (Dox) nor ponasterone was added when neither Rac1-V12 nor dominant negativve IκB [IκB(DN)] was expressed as a negative control.
- Supplemental Figure B
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Only 1 μg/ml Dox was added to the transfected culture to induce the expression of Rac1-V12.
- Supplemental Figure C
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1 μg/ml Dox and 5 μM ponasterone were added to the transfected culture to express both Rac1-V12 and IκB(DN).
