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Am J Physiol Cell Physiol 296: C1271-C1278, 2009. First published April 8, 2009; doi:10.1152/ajpcell.00088.2009
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MEMBRANE TRANSPORTERS, ION CHANNELS, AND PUMPS

Kv2.1 and silent Kv subunits underlie the delayed rectifier K+ current in cultured small mouse DRG neurons

Elke Bocksteins,1 Adam L. Raes,1 Gerda Van de Vijver,2 Tine Bruyns,1 Pierre-Paul Van Bogaert,2 and Dirk J. Snyders1

1Laboratory for Molecular Biophysics, Physiology and Pharmacology, Department of Biomedical Sciences, and 2Laboratory for Cardiovascular Research, Institute Born-Bunge, University of Antwerp, Antwerpen, Belgium

Submitted 26 February 2009 ; accepted in final form 6 April 2009

Silent voltage-gated K+ (Kv) subunits interact with Kv2 subunits and primarily modulate the voltage dependence of inactivation of these heterotetrameric channels. Both Kv2 and silent Kv subunits are expressed in the mammalian nervous system, but little is known about their expression and function in sensory neurons. This study reports the presence of Kv2.1, Kv2.2, and silent subunit Kv6.1, Kv8.1, Kv9.1, Kv9.2, and Kv9.3 mRNA in mouse dorsal root ganglia (DRG). Immunocytochemistry confirmed the protein expression of Kv2.x and Kv9.x subunits in cultured small DRG neurons. To investigate if Kv2 and silent Kv subunits are underlying the delayed rectifier K+ current (IK) in these neurons, Kv2-mediated currents were isolated by the extracellular application of rStromatoxin-1 (ScTx) or by the intracellular application of Kv2 antibodies. Both ScTx- and anti-Kv2.1-sensitive currents displayed two components in their voltage dependence of inactivation. Together, both components accounted for approximately two-thirds of IK. A comparison with results obtained in heterologous expression systems suggests that one component reflects homotetrameric Kv2.1 channels, whereas the other component represents heterotetrameric Kv2.1/silent Kv channels. These observations support a physiological role for silent Kv subunits in small DRG neurons.

voltage-gated K+ channels; dorsal root ganglia neurons; rStromatoxin-1; Kv2.1 antibodies


Address for reprint requests and other correspondence: D. J. Snyders, Laboratory for Molecular Biophysics, Physiology and Pharmacology, Dept. of Biomedical Sciences, Univ. of Antwerp, Universiteitsplein 1, Antwerp 2610, Belgium (e-mail: dirk.snyders{at}ua.ac.be)






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