Am J Physiol Cell Physiol Ad Instruments
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Cell Physiol 296: C441-C452, 2009. First published December 17, 2008; doi:10.1152/ajpcell.00324.2008
0363-6143/09 $8.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental Figures
Right arrow RETRACTION
Right arrow All Versions of this Article:
296/3/C441    most recent
00324.2008v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lin, J.
Right arrow Articles by Low, W. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lin, J.
Right arrow Articles by Low, W. C.

NERVOUS SYSTEM CELL BIOLOGY

Directed differentiation of mouse cochlear neural progenitors in vitro

Jizhen Lin,1,4,* Ling Feng,1,* Yuki Hamajima,1 Masahiro Komori,1 Terry C. Burns,2,3,4 Shinji Fukudome,1 John Anderson,1,2 Dong Wang,5 Catherine M. Verfaillie,4 and Walter C. Low2,3,4

Departments of 1Otolaryngology and 2Neurosurgery, 3Graduate Program in Neuroscience, 4Stem Cell Institute, and 5Bioengineering, University of Minnesota, Minneapolis, Minnesota

Submitted 18 June 2008 ; accepted in final form 7 December 2008

Multipotent cochlear neural progenitors (CNPs) in the organ of Corti hold the promise for cell replacement in degenerative hearing disorders. However, not much is known about the CNPs and the specific conditions for their differentiation. Here we isolate the CNPs from the postnatal day 1 organ of Corti in mice and demonstrate their capability to self-renew and to differentiate into hair cell-like and neuronal cell-like phenotypes under the guidance of sonic hedgehog (SHH), epidermal growth factor (EGF), retinoic acid (RA), and brain-derived neurotrophic factor (BDNF), herein termed SERB (abbreviation of SHH, EGF, RA, and BDNF) in an asymmetric or symmetric manner from clonal isolates. Differentiation of CNPs into hair cells by SERB was dependent on the ERK signaling pathway, whereas the differentiation of CNPs into neurons by SERB was not. This work develops a new in vitro methodology for the maintenance and self-regeneration of CNPs for future design of regenerative strategies for hearing disorders.

growth factor; proliferation


Address for reprint requests and other correspondence: J. Lin, 216 Lions Research Bldg., Univ. of Minnesota, 2001 Sixth St. S.E., Minneapolis, MN 55455 (e-mail: linxx004{at}tc.umn.edu)






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online
Copyright © 2009 by the American Physiological Society.