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GROWTH, DIFFERENTIATION, AND APOPTOSIS

The extreme COOH terminus of the retinoblastoma tumor suppressor protein pRb is required for phosphorylation on Thr-373 and activation of E2F

¹Department of Pharmacological Sciences at Saint Louis University, St. Louis, Missouri; and ²Department of Sciences at John Jay College of Criminal Justice, The City University of New York, New York

Submitted 8 June 2008 ; accepted in final form 27 August 2008

The retinoblastoma protein pRb plays a pivotal role in G₁- to S-phase cell cycle progression and is among the most frequently mutated gene products in human cancer. Although much focus has been placed on understanding how the A/B pocket and COOH-terminal domain of pRb cooperate to relieve transcriptional repression of E2F-responsive genes, comparatively little emphasis has been placed on the function of the NH₂-terminal region of pRb and the interaction of the multiple domains of pRb in the full-length context. Using "reverse mutational analysis" of Rb^CDK (a dominantly active repressive allele of Rb), we have previously shown that restoration of Thr-373 is sufficient to render Rb^CDK sensitive to inactivation via cyclin-CDK phosphorylation. This suggests that the NH₂-terminal region plays a more critical role in pRb regulation than previously thought. In the present study, we have expanded this analysis to include additional residues in the NH₂-terminal region of pRb and further establish that the mechanism of pRb inactivation by Thr-373 phosphorylation is through the dissociation of E2F. Most surprisingly, we further have found that removal of the COOH-terminal domain of either RbCDK^+T373 or wild-type pRb yields a functional allele that cannot be inactivated by phosphorylation and is repressive of E2F activation and S-phase entry. Our data demonstrate a novel function for the NH₂-terminal domain of pRb and the necessity for cooperation of multiple domains for proper pRb regulation.

cyclin; cell cycle