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EXTRACELLULAR MATRIX, CELL INTERACTIONS

₄β₁-Integrin regulates directionally persistent cell migration in response to shear flow stimulation

¹Department of Cell Biology, Johns Hopkins University School of Medicine, and ²Department of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, Maryland

Submitted 22 March 2008 ; accepted in final form 15 May 2008

₄β₁-Integrin plays a pivotal role in cell migration in vivo. This integrin has been shown to regulate the front-back polarity of migrating cells via localized inhibition of ₄-integrin/paxillin binding by phosphorylation at the ₄-integrin cytoplasmic tail. Here, we demonstrate that ₄β₁-integrin regulates directionally persistent cell migration via a more complex mechanism in which ₄-integrin phosphorylation and paxillin binding act via both cooperative and independent pathways. We show that, in response to shear flow, ₄β₁-integrin binding to the CS-1 region of fibronectin was necessary and sufficient to promote directionally persistent cell migration when this integrin was ectopically expressed in CHO cells. Under shear flow, the ₄β₁-integrin-expressing cells formed a fan shape with broad lamellipodia at the front and retracted trailing edges at the back. This "fanning" activity was enhanced by disrupting paxillin binding alone and inhibited by disrupting phosphorylation alone or together with disrupting paxillin binding. Notably, the phosphorylation-disrupting mutation and the double mutation resulted in the formation of long trailing tails, suggesting that ₄-integrin phosphorylation is required for trailing edge retraction/detachment independent of paxillin binding. Furthermore, the stable polarity and directional persistence of shear flow-stimulated cells were perturbed by the double mutation but not the single mutations alone, indicating that paxillin binding and ₄-integrin phosphorylation can facilitate directionally persistent cell migration in an independent and compensatory manner. These findings provide a new insight into the mechanism by which integrins regulate directionally persistent cell migration.

lamellipodia; fibronectin; shear stress