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RECEPTORS AND SIGNAL TRANSDUCTION

NO-induced regulation of human trabecular meshwork cell volume and aqueous humor outflow facility involve the BK_Ca ion channel

University of Florida, College of Pharmacy, Department of Pharmacodynamics, Gainesville, Florida

Submitted 13 August 2007 ; accepted in final form 1 April 2008

Nitric oxide (NO) donors decrease intraocular pressure (IOP) by increasing aqueous outflow facility in the trabecular meshwork (TM) and/or Schlemm's canal. However, the cellular mechanisms are unknown. Cellular mechanisms known to regulate outflow facility include changes in cell volume and cellular contractility. In this study, we investigated the effects of NO donors on outflow facility and NO-induced effects on TM cell volume. We tested the involvement of soluble guanylate cyclase (sGC), cGMP, PKG, and the large-conductance Ca²⁺-activated K⁺ (BK_Ca) channel using inhibitors and activators. Cell volume was measured using calcein AM fluorescent dye, detected by confocal microscopy, and quantified using NIH ImageJ software. An anterior segment organ perfusion system measured outflow facility. NO increased outflow facility in porcine eye anterior segments (0.4884–1.3956 µl·min^–1·mmHg^–1) over baseline (0.2373–0.5220 µl·min^–1·mmHg^–1) within 10 min of drug application. These NO-induced increases in outflow facility were inhibited by the the BK_Ca channel inhibitor IBTX. Exposure of TM cells to NO resulted in a 10% decrease in cell volume, and these decreases were abolished by the sGC inhibitor 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one and IBTX, suggesting the involvement of sGC and K⁺ eflux, respectively. NO-induced decreases in cell volume were mimicked by 8-Br-cGMP and abolished by the PKG inhibitor (RP)-8-Br-PET-cGMP-S, suggesting the involvement cGMP and PKG. Additionally, the time course for NO-induced decreases in TM cell volume correlated with NO-induced increases in outflow facility, suggesting that the NO-induced alterations in cell volume may influence outflow facility.

eye; signal transduction; cGMP; protein kinase G; soluble guanylate cyclase