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This Article Full Text Full Text (PDF) All Versions of this Article: 293/5/C1627    most recent 00122.2007v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (3) Citing Articles via Google Scholar Google Scholar Articles by Li, L. Articles by Song, B. Search for Related Content PubMed PubMed Citation Articles by Li, L. Articles by Song, B.

MUSCLE CELL BIOLOGY AND CELL MOTILITY

Changes of gap junctional cell-cell communication in overactive detrusor in rats

¹Center of Urology, Southwest Hospital, Third Military Medical University, Chongqing, China; ²Department of Biomedicine and Surgery, University of Linköping, Linköping, Sweden; and ³Center of Oncology, Xinqiao Hospital, Third Military Medical University, Chongqing, China

Submitted 29 March 2007 ; accepted in final form 29 August 2007

To evaluate the changes in intercellular communication through gap junctions in detrusor overactivity (DO), we studied 23 adult female Wistar rats with DO after partial outflow obstruction (DO group) and 13 sham-operated rats (control group). The two groups were compared by means of urodynamics, light and electron microscopy, expression of Cx40, Cx43, and Cx45 mRNA genes with RT-PCR, Cx43 protein with Western blot analysis, and functional intercellular communication with scrape loading dye transfer (SLDT) and fluorescence recovery after photobleaching (FRAP). The number of gap junctions and the expression of connexin mRNA and Cx43 protein were increased in DO rats, and intercellular communication through gap junctions increased after 6 wk of partial outflow obstruction as assessed with SLDT and FRAP techniques. The findings provide a theoretical rationale for using Cx43 antagonists and gap junction inhibitors in the treatment of patients with overactive detrusor secondary to partial bladder outflow obstruction.

partial bladder outflow obstruction; gap junction

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