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Am J Physiol Cell Physiol 293: C1627-C1635, 2007. First published September 13, 2007; doi:10.1152/ajpcell.00122.2007
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MUSCLE CELL BIOLOGY AND CELL MOTILITY

Changes of gap junctional cell-cell communication in overactive detrusor in rats

Longkun Li,1 Chonghe Jiang,2 Ping Hao,3 Weibing Li,1 Caiping Song,1 and Bo Song1

1Center of Urology, Southwest Hospital, Third Military Medical University, Chongqing, China; 2Department of Biomedicine and Surgery, University of Linköping, Linköping, Sweden; and 3Center of Oncology, Xinqiao Hospital, Third Military Medical University, Chongqing, China

Submitted 29 March 2007 ; accepted in final form 29 August 2007

To evaluate the changes in intercellular communication through gap junctions in detrusor overactivity (DO), we studied 23 adult female Wistar rats with DO after partial outflow obstruction (DO group) and 13 sham-operated rats (control group). The two groups were compared by means of urodynamics, light and electron microscopy, expression of Cx40, Cx43, and Cx45 mRNA genes with RT-PCR, Cx43 protein with Western blot analysis, and functional intercellular communication with scrape loading dye transfer (SLDT) and fluorescence recovery after photobleaching (FRAP). The number of gap junctions and the expression of connexin mRNA and Cx43 protein were increased in DO rats, and intercellular communication through gap junctions increased after 6 wk of partial outflow obstruction as assessed with SLDT and FRAP techniques. The findings provide a theoretical rationale for using Cx43 antagonists and gap junction inhibitors in the treatment of patients with overactive detrusor secondary to partial bladder outflow obstruction.

partial bladder outflow obstruction; gap junction


Address for reprint requests and other correspondence: B. Song, Center of Urology, Southwest Hospital, Third Military Medical Univ., Chongqing 400038, China (e-mail: songbo_cq{at}yahoo.com)






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