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Am J Physiol Cell Physiol 293: C1561-C1567, 2007. First published August 15, 2007; doi:10.1152/ajpcell.00240.2007
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MEMBRANE TRANSPORTERS, ION CHANNELS, AND PUMPS

Functional ion channels in mouse bone marrow mesenchymal stem cells

Rong Tao,1 Chu-Pak Lau,1 Hung-Fat Tse,1 and Gui-Rong Li1,2

1Department of Medicine and Research Center of Heart, Brain, Hormone and Healthy Aging, and 2Department of Physiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong SAR, China

Submitted 7 June 2007 ; accepted in final form 7 August 2007

Bone marrow mesenchymal stem cells (MSCs) are used as a cell source for cardiomyoplasty; however, the cellular electrophysiological properties are not fully understood. The present study was to investigate the functional ionic channels in undifferentiated mouse bone marrow MSCs using whole cell patch-voltage clamp technique, RT-PCR, and Western immunoblotting analysis. We found that three types of ionic currents were present in mouse MSCs, including a Ca2+-activated K+ current (IKCa), an inwardly rectifying K+ current (IKir), and a chloride current (ICl). IKir was inhibited by Ba2+, and IKCa was activated by the Ca2+ ionophore A-23187 and inhibited by the intermediate-conductance IKCa channel blocker clotrimazole. ICl was activated by hyposmotic (0.8 T) conditions and inhibited by the chloride channel blockers DIDS and NPPB. The corresponding ion channel genes and proteins, KCa3.1 for IKCa, Kir2.1 for IKir, and Clcn3 for ICl, were confirmed by RT-PCR and Western immunoblotting analysis in mouse MSCs. These results demonstrate that three types of functional ion channel currents (i.e., IKir, IKCa, and ICl) are present in mouse bone marrow MSCs.

inward rectifier potassium current; intermediate-conductance calcium-activated potassium current; volume-sensitive chloride current


Address for reprint requests and other correspondence: G.-R. Li, L8-01, Laboratory Block, Faculty of Medicine Bldg., The Univ. of Hong Kong, 21 Sassoon Road, Pokfulam, Hong Kong SAR, China (e-mail: grli{at}hkucc.hku.hk)




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R. Tao, C.-P. Lau, H.-F. Tse, and G.-R. Li
Regulation of cell proliferation by intermediate-conductance Ca2+-activated potassium and volume-sensitive chloride channels in mouse mesenchymal stem cells
Am J Physiol Cell Physiol, November 1, 2008; 295(5): C1409 - C1416.
[Abstract] [Full Text] [PDF]


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