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Am J Physiol Cell Physiol 293: C1532-C1538, 2007. First published August 8, 2007; doi:10.1152/ajpcell.00065.2007
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GROWTH, DIFFERENTIATION, AND APOPTOSIS

Human adipose-derived adult stem cells upregulate palladin during osteogenesis and in response to cyclic tensile strain

Michelle E. Wall,1,* Andrew Rachlin,2,* Carol A. Otey,2 and Elizabeth G. Loboa1

1Joint Department of Biomedical Engineering, University of North Carolina at Chapel Hill and North Carolina State University, Raleigh; and 2Department of Cell and Molecular Physiology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina

Submitted 16 February 2007 ; accepted in final form 6 August 2007

Cell morphology may be an important stimulus during differentiation of human adipose-derived adult stem (hADAS) cells, but there are limited studies that have investigated the role of the cytoskeleton or associated proteins in hADAS cells undergoing differentiation. Palladin is an actin-associated protein that plays an integral role in focal adhesion and cytoskeleton organization. In this study we show that palladin was expressed by hADAS cells and was modulated during osteogenic differentiation and in response to cyclic tensile strain. Human ADAS cells expressed the 90- and 140-kDa palladin isoforms and upregulated expression of both isoforms after culture in conditions that promoted osteogenesis. Palladin mRNA expression levels were also increased in hADAS cells subjected to cyclic tensile strain. Knockdown of the palladin gene during osteogenesis resulted in decreased actin stress fibers and decreased protein levels of Eps8, an epidermal growth factor receptor tyrosine kinase that colocalizes with actin. Silencing the palladin gene, however, did not affect hADAS cells' commitment down the osteogenic lineage.

adipose-derived adult stem cells; mechanobiology; Eps8; actin cytoskeleton; mesenchymal stem cells


Address for reprint requests and other correspondence: E. G. Loboa, Joint Dept. of Biomedical Engineering at UNC-Chapel Hill and NC State Univ., 2142 Burlington Laboratories, Campus Box 7115, NC State Univ., Raleigh, NC 27695-7115 (e-mail: egloboa{at}ncsu.edu)






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