HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: 293/3/C1181    most recent 00066.2007v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Ross, S. B. Articles by Benos, D. J. Search for Related Content PubMed PubMed Citation Articles by Ross, S. B. Articles by Benos, D. J.

REPORT

MEMBRANE TRANSPORTERS, ION CHANNELS, AND PUMPS

Amiloride-sensitive Na⁺ channels contribute to regulatory volume increases in human glioma cells

Department of Physiology and Biophysics, University of Alabama at Birmingham, Birmingham, Alabama

Submitted 16 February 2007 ; accepted in final form 28 June 2007

ABSTRACT

Despite intensive research, brain tumors remain among the most difficult type of malignancies to treat, due largely to their diffusely invasive nature and the associated difficulty of adequate surgical resection. To migrate through the brain parenchyma and to proliferate, glioma cells must be capable of significant changes in shape and volume. We have previously reported that glioma cells express an amiloride- and psalmotoxin-sensitive cation conductance that is not found in normal human astrocytes. In the present study, we investigated the potential role of this ion channel to mediate regulatory volume increase in glioma cells. We found that the ability of the cells to volume regulate subsequent to cell shrinkage by hyperosmolar solutions was abolished by both amiloride and psalmotoxin 1. This toxin is thought to be a specific peptide inhibitor of acid-sensing ion channel (ASIC1), a member of the Deg/ENaC superfamily of cation channels. We have previously shown this toxin to be an effective blocker of the glioma cation conductance. Our data suggest that one potential role for this conductance may be to restore cell volume during the cell's progression thorough the cell cycle and while the tumor cell migrates within the interstices of the brain.

brain tumor; sodium; epithelial sodium channel; acid-sensing ion channel; psalmotoxin

This article has been cited by other articles:

				N. Kapoor, R. Bartoszewski, Y. J. Qadri, Z. Bebok, J. K. Bubien, C. M. Fuller, and D. J. Benos Knockdown of ASIC1 and Epithelial Sodium Channel Subunits Inhibits Glioblastoma Whole Cell Current and Cell Migration J. Biol. Chem., September 4, 2009; 284(36): 24526 - 24541. [Abstract] [Full Text] [PDF]

				E. Bashari, Y. J. Qadri, Z.-H. Zhou, N. Kapoor, S. J. Anderson, R. H. Meltzer, C. M. Fuller, and D. J. Benos Two PKC consensus sites on human acid-sensing ion channel 1b differentially regulate its function Am J Physiol Cell Physiol, February 1, 2009; 296(2): C372 - C384. [Abstract] [Full Text] [PDF]

				E. K. Hoffmann, I. H. Lambert, and S. F. Pedersen Physiology of Cell Volume Regulation in Vertebrates Physiol Rev, January 1, 2009; 89(1): 193 - 277. [Abstract] [Full Text] [PDF]