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Am J Physiol Cell Physiol 293: C761-C771, 2007. First published May 30, 2007; doi:10.1152/ajpcell.00043.2007
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CELLULAR METABOLISM

Mitochondrial alterations in human gastric carcinoma cell line

Hyoung Kyu Kim,1 Won Sun Park,1 Sung Hyun Kang,1 Mohamad Warda,1,2 Nari Kim,1 Jae-Hong Ko,1 Abd El-bary Prince, and Jin Han1

1Mitochondrial Signaling Laboratory, Mitochondria Research Group, Department of Physiology and Biophysics, College of Medicine, Biohealth Products Research Center, Cardiovascular and Metabolic Disease Research Center, Inje University, Busan, Korea; and 2Department of Biochemistry, Faculty of Veterinary Medicine, Cairo University, Cairo, Egypt

Submitted 31 January 2007 ; accepted in final form 24 May 2007

We compared mitochondrial function, morphology, and proteome in the rat normal gastric cell line RGM-1 and the human gastric cancer cell line AGS. Total numbers and cross-sectional sizes of mitochondria were smaller in AGS cells. Mitochondria in AGS cells were deformed and consumed less oxygen. Confocal microscopy indicated that the mitochondrial inner membrane potential was hyperpolarized and the mitochondrial Ca2+ concentration was elevated in AGS cells. Interestingly, two-dimensional electrophoresis proteomics on the mitochondria-enriched fraction revealed high expression of four mitochondrial proteins in AGS cells: ubiquinol-cytochrome c reductase, mitochondrial short-chain enoyl-coenzyme A hydratase-1, heat shock protein 60, and mitochondria elongation factor Tu. The results provide clues as to the mechanism of the mitochondrial changes in cancer at the protein level and may serve as potential cancer biomarkers in mitochondria.

two-dimensional gel electrophoresis proteomics; biomarker; cancer


Address for reprint requests and other correspondence: J. Han, Mitochondrial Signaling Laboratory, Mitochondria Research Group, Dept. of Physiology and Biophysics, College of Medicine, Biohealth Products Research Center, Cardiovascular and Metabolic Disease Center, Inje Univ. 633-165 Gaegeum-Dong, Busanjin-Gu, Busan 613-735, Korea (e-mail: phyhanj{at}ijnc.inje.ac.kr)






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