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MUSCLE CELL BIOLOGY AND CELL MOTILITY

Carbonic anhydrase XIV in skeletal muscle: subcellular localization and function from wild-type and knockout mice

¹Zentrum Physiologie and ²Zentrum Biochemie, Medizinische Hochschule Hannover, Hannover, Germany; and ³Edward A. Doisy Department of Biochemistry and Molecular Biology, Saint Louis University School of Medicine, St. Louis, Missouri

Submitted 9 February 2007 ; accepted in final form 18 April 2007

The expression of carbonic anhydrase (CA) XIV was investigated in mouse skeletal muscles. Sarcoplasmic reticulum (SR) and sarcolemmal (SL) membrane fractions were isolated from wild-type (WT) and CA XIV knockout (KO) mice. The CA XIV protein of 54 kDa was present in SR and SL membrane fractions as shown by Western blot analysis. CA activity measurements of WT and KO membrane fractions showed that CA XIV accounts for 50% and 66% of the total CA activities determined in the SR and SL fractions, respectively. This indicates the presence of at least one other membrane-associated CA isoform in these membranes, e.g., CA IV, CA IX, or CA XII. Muscle fibers of the extensor digitorum longus (EDL) muscle were immunostained with anti-CA XIV/FITC and anti-sarco(endo)plasmic reticulum Ca²⁺-ATPase 1/TRITC, with anti-CA XIV/FITC and anti-ryanodine receptor/TRITC, or with anti-CA XIV/FITC and anti-monocarboxylate transporter-4/TRITC. CA XIV was expressed in the plasma membrane and in the longitudinal SR but not in the terminal SR. Isometric contraction measurements of single twitches and tetani and a fatigue protocol applied to fiber bundles of the fast-twitch EDL and of the slow-twitch soleus muscle from WT and KO mice showed that the lack of SR membrane-associated CA XIV did not affect maximum force, rise and relaxation times, and fatigue behavior. Thus, it is concluded that a reduction of the total SR CA activity by 50% in CA XIV KO mice does not lead to an impairment of SR function.

sarcoplasmic reticulum; sarcolemma; isometric contraction; Ca²⁺-ATPase; ryanodine receptor

This article has been cited by other articles:

				R. J. Scheibe, K. Mundhenk, T. Becker, J. Hallerdei, A. Waheed, G. N. Shah, W. S. Sly, G. Gros, and P. Wetzel Carbonic anhydrases IV and IX: subcellular localization and functional role in mouse skeletal muscle Am J Physiol Cell Physiol, February 1, 2008; 294(2): C402 - C412. [Abstract] [Full Text] [PDF]