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Am J Physiol Cell Physiol 291: C687-C698, 2006. First published April 19, 2006; doi:10.1152/ajpcell.00510.2005
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NERVOUS SYSTEM CELL BIOLOGY

K+ channel KV3.1 associates with OSP/claudin-11 and regulates oligodendrocyte development

Seema Tiwari-Woodruff, Luis Beltran-Parrazal, Andrew Charles, Thomas Keck, Trung Vu, and Jeff Bronstein

UCLA Multiple Sclerosis Program, Department of Neurology, David Geffen School of Medicine at UCLA, Los Angeles, California

Submitted 12 October 2005 ; accepted in final form 12 April 2006

K+ channels are differentially expressed throughout oligodendrocyte (Olg) development. KV1 family voltage-sensitive K+ channels have been implicated in proliferation and migration of Olg progenitor cell (OPC) stage, and inward rectifier K+ channels (KIR)4.1 are required for OPC differentiation to myelin-forming Olg. In this report we have identified a Shaw family K+ channel, KV3.1, that is involved in proliferation and migration of OPC and axon myelination. Application of anti-KV3.1 antibody or knockout of Kv3.1 gene decreased the sustained K+ current component of OPC by 50% and 75%, respectively. In functional assays block of KV3.1-specific currents or knockout of Kv3.1 gene inhibited proliferation and migration of OPC. Adult Kv3.1 gene-knockout mice had decreased diameter of axons and decreased thickness of myelin in optic nerves compared with age-matched wild-type littermates. Additionally, KV3.1 was identified as an associated protein of Olg-specific protein (OSP)/claudin-11 via yeast two-hybrid analysis, which was confirmed by coimmunoprecipitation and coimmunohistochemistry. In summary, the KV3.1 K+ current accounts for a significant component of the total K+ current in cells of the Olg lineage and, in association with OSP/claudin-11, plays a significant role in OPC proliferation and migration and myelination of axons.

membrane potential; tight junction; myelin; progenitor cell


Address for reprint requests and other correspondence: S. K. Tiwari-Woodruff, Glia Biology Group, UCLA Multiple Sclerosis Program, Dept. of Neurology, NRB1, 635 Charles Young Dr. S., Los Angeles, CA 90095 (e-mail: seemaw{at}ucla.edu)






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