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This Article Full Text Full Text (PDF) All Versions of this Article: 291/4/C589    most recent 00623.2005v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Berfield, A. K. Articles by Abrass, C. K. Search for Related Content PubMed PubMed Citation Articles by Berfield, A. K. Articles by Abrass, C. K.

EXTRACELLULAR MATRIX, CELL INTERACTIONS

Rat glomerular mesangial cells require laminin-9 to migrate in response to insulin-like growth factor binding protein-5

¹Department of Medicine, School of Medicine, University of Washington; and ²Veterans Affairs Puget Sound Health Care System, Seattle, Washington

Submitted 13 December 2005 ; accepted in final form 25 April 2006

Temporal and spatial differences in extracellular matrix play critical roles in cell proliferation, differentiation and migration. Different migratory stimuli use different substrates and receptors to achieve cell migration. To understand the mechanism of insulin-like growth factor binding protein-5 (IGFBP-5)-induced migration in mesangial cells, the roles of integrins and substrates were examined. IGFBP-5 induced an increase in mRNA expression for laminin (LN) chains lama4, lamb2, and lamc1, suggesting that LN-9 might be required for migration. Antibodies to the LN₄ and LN₂ chains, but not LN₁, blocked IGFBP-5-induced migration. Anti-sense morpholino oligonucleotide inhibition of expression of LN₄ substantially reduced expression of LN-8/9 (₄₁₁/₄₂₁, 411/421) and prevented IGFBP-5-induced migration. Anti-sense inhibition of lamb2 reduced expression of LN-9. Absence of LN-9 prevented IGFBP-5-induced migration, which was not preserved by continued expression of LN-8. The requirement for LN-9 was further supported by studies of T98G cells, which express predominantly LN-8. IGFBP-5 had little effect on migration in these cells, but increased migration when T98G cells were plated on LN-8/9. IGFBP-5-mediated mesangial cell migration was inhibited by antibodies that block attachment to ₆₁-integrins but was unaffected by antibodies and disintegrins that block binding to other integrins. Furthermore, in cells with anti-sense inhibited expression of LN-9, integrin ₆₁ was no longer detected on the cell surface. These studies suggest the specificity of mechanisms of migration induced by specific stimuli and for the first time demonstrate a unique function for LN-9 in mediating IGFBP-5-induced migration.

migration; integrins; extracellular matrix