HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: 290/4/C972    most recent 00136.2005v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (3) Citing Articles via Google Scholar Google Scholar Articles by Chao, J.-T. Articles by Wilson, E. Search for Related Content PubMed PubMed Citation Articles by Chao, J.-T. Articles by Wilson, E.

VASCULAR BIOLOGY

Modulation of ₇-integrin-mediated adhesion and expression by platelet-derived growth factor in vascular smooth muscle cells

¹Division of Vascular Biology, Cardiovascular Research Institute, The Texas A&M University System Health Science Center, College Station, Texas; ²Department of Cell and Structural Biology, University of Illinois, Urbana, Illinois; and ³Department of Biochemistry and Molecular Biology, University of Louisville, Louisville, Kentucky

Submitted 23 March 2005 ; accepted in final form 2 November 2005

We showed previously that the expression of ₇-integrin in aortic vascular smooth muscle cells (VSMC) is enhanced in a rat model of atherosclerosis. In the present study, we investigated the effects of platelet-derived growth factor (PDGF) on ₇-integrin expression and VSMC adhesion and migration. Expression of the ₇-integrin gene was determined by real-time RT-PCR, whereas protein levels were determined by fluorescence-activated cell sorting analysis. PDGF increased ₇ cell surface protein expression (12 and 24 h: 3.3 ± 0.8- and 3.6 ± 0.4-fold, P < 0.05 vs. control) and mRNA levels (24 h: 3.1-fold, P < 0.05 vs. control) in a time-dependent manner. Actinomycin D and cycloheximide attenuated PDGF-induced increases in ₇-integrin, indicating the involvement of de novo mRNA and protein synthesis. Treatment with the MAPK inhibitors PD-98059, SP-600125, and SB-203580 attenuated PDGF-induced increases in mRNA. In contrast, PD-98059 and SP-600125, but not SB-203580, attenuated PDGF-induced increases in cell surface protein levels. PDGF-treated VSMC adhered to laminin more efficiently (42 ± 6% increase, P < 0.01), and this increase was partially inhibited by anti-₇-integrin function-blocking antibody. However, PDGF did not alter migration on laminin, and there was no effect of the anti-₇-integrin function-blocking antibody on basal or PDGF-stimulated migration. Immunofluorescence imaging revealed an increase in ₇-integrin distribution along the stress fibers. Together, these observations indicate that PDGF enhances ₇-integrin expression in VSMC and promotes ₇-integrin-mediated adhesion to laminin.

vascular injury; laminin; mitogen-activated protein kinase

This article has been cited by other articles:

				R. Silva, G. D'Amico, K. M. Hodivala-Dilke, and L. E. Reynolds Integrins: The Keys to Unlocking Angiogenesis Arterioscler. Thromb. Vasc. Biol., October 1, 2008; 28(10): 1703 - 1713. [Abstract] [Full Text] [PDF]

				J. V. Welser, N. Lange, C. A. Singer, M. Elorza, P. Scowen, K. D. Keef, W. T. Gerthoffer, and D. J. Burkin Loss of the {alpha}7 Integrin Promotes Extracellular Signal-Regulated Kinase Activation and Altered Vascular Remodeling Circ. Res., September 28, 2007; 101(7): 672 - 681. [Abstract] [Full Text] [PDF]