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Am J Physiol Cell Physiol 290: C371-C378, 2006. First published September 7, 2005; doi:10.1152/ajpcell.00281.2005
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MEMBRANE TRANSPORTERS, ION CHANNELS, AND PUMPS

Proton pump-rich cell secretes acid in skin of zebrafish larvae

Li-Yih Lin,1,2 Jiun-Lin Horng,1,3 Joseph G. Kunkel,4 and Pung-Pung Hwang1,3

1Institute of Cellular and Organismic Biology, Academia Sinica, Taipei, 2Department of Physical Therapy, Shu-Zen College of Medicine and Management, Kaohsiung, and 3Graduate Institute of Life Sciences, National Defense Medical Center, Taipei, Taiwan, Republic of China; and 4Department of Biology, University of Massachusetts, Amherst, Massachusetts

Submitted 13 June 2005 ; accepted in final form 5 September 2005

The mammalian kidney excretes its metabolic acid load through the proton-transporting cells, intercalated cells, in the distal nephron and collecting duct. Fish excrete acid through external organs, gill, or skin; however, the cellular function is still controversial. In this study, molecular and electrophysiological approaches were used to identify a novel cell type secreting acid in skin of zebrafish (Danio rerio) larvae. Among keratinocytes covering the larval surface, novel proton-secreting ionocytes, proton pump (H+-ATPase)-rich cells, were identified to generate strong outward H+ flux. The present work demonstrates for the first time, with a noninvasive technique, H+-secreting cells in an intact animal model, the zebrafish, showing it to be a suitable model in which to study the functions of vertebrate transporting epithelia in vivo.

hydrogen-adenosinetriphosphatase; ionocytes; epithelial transport; ion-selective electrode


Address for reprint requests and other correspondence: P.-P. Hwang, Institute of Cellular and Organismic Biology, Academia Sinica, Nankang, Taipei, Taiwan, Republic of China (e-mail: pphwang{at}gate.sinica.edu.tw)




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