| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
MEMBRANE TRANSPORTERS, ION CHANNELS, AND PUMPS
1Institute of Cellular and Organismic Biology, Academia Sinica, Taipei, 2Department of Physical Therapy, Shu-Zen College of Medicine and Management, Kaohsiung, and 3Graduate Institute of Life Sciences, National Defense Medical Center, Taipei, Taiwan, Republic of China; and 4Department of Biology, University of Massachusetts, Amherst, Massachusetts
Submitted 13 June 2005 ; accepted in final form 5 September 2005
The mammalian kidney excretes its metabolic acid load through the proton-transporting cells, intercalated cells, in the distal nephron and collecting duct. Fish excrete acid through external organs, gill, or skin; however, the cellular function is still controversial. In this study, molecular and electrophysiological approaches were used to identify a novel cell type secreting acid in skin of zebrafish (Danio rerio) larvae. Among keratinocytes covering the larval surface, novel proton-secreting ionocytes, proton pump (H+-ATPase)-rich cells, were identified to generate strong outward H+ flux. The present work demonstrates for the first time, with a noninvasive technique, H+-secreting cells in an intact animal model, the zebrafish, showing it to be a suitable model in which to study the functions of vertebrate transporting epithelia in vivo.
hydrogen-adenosinetriphosphatase; ionocytes; epithelial transport; ion-selective electrode
This article has been cited by other articles:
|
|
 |
|
  P.-P. Hwang Ion uptake and acid secretion in zebrafish (Danio rerio) J. Exp. Biol., June 1, 2009; 212(11): 1745 - 1752. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B.-K. Liao, R.-D. Chen, and P.-P. Hwang Expression regulation of Na+-K+-ATPase {alpha}1-subunit subtypes in zebrafish gill ionocytes Am J Physiol Regulatory Integrative Comp Physiol, June 1, 2009; 296(6): R1897 - R1906. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.-F. Wang, Y.-C. Tseng, J.-J. Yan, J. Hiroi, and P.-P. Hwang Role of SLC12A10.2, a Na-Cl cotransporter-like protein, in a Cl uptake mechanism in zebrafish (Danio rerio) Am J Physiol Regulatory Integrative Comp Physiol, May 1, 2009; 296(5): R1650 - R1660. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. S. Flynt, E. J. Thatcher, K. Burkewitz, N. Li, Y. Liu, and J. G. Patton miR-8 microRNAs regulate the response to osmotic stress in zebrafish embryos J. Cell Biol., April 6, 2009; 185(1): 115 - 127. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Inokuchi, J. Hiroi, S. Watanabe, P.-P. Hwang, and T. Kaneko Morphological and functional classification of ion-absorbing mitochondria-rich cells in the gills of Mozambique tilapia J. Exp. Biol., April 1, 2009; 212(7): 1003 - 1010. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-L. Horng, L.-Y. Lin, and P.-P. Hwang Functional regulation of H+-ATPase-rich cells in zebrafish embryos acclimated to an acidic environment Am J Physiol Cell Physiol, April 1, 2009; 296(4): C682 - C692. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W.-J. Chang, J.-L. Horng, J.-J. Yan, C.-D. Hsiao, and P.-P. Hwang The transcription factor, glial cell missing 2, is involved in differentiation and functional regulation of H+-ATPase-rich cells in zebrafish (Danio rerio) Am J Physiol Regulatory Integrative Comp Physiol, April 1, 2009; 296(4): R1192 - R1201. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. K. Parks, M. Tresguerres, and G. G. Goss Cellular mechanisms of Cl- transport in trout gill mitochondrion-rich cells Am J Physiol Regulatory Integrative Comp Physiol, April 1, 2009; 296(4): R1161 - R1169. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T.-H. Shih, J.-L. Horng, P.-P. Hwang, and L.-Y. Lin Ammonia excretion by the skin of zebrafish (Danio rerio) larvae Am J Physiol Cell Physiol, December 1, 2008; 295(6): C1625 - C1632. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Hiroi, S. Yasumasu, S. D. McCormick, P.-P. Hwang, and T. Kaneko Evidence for an apical Na-Cl cotransporter involved in ion uptake in a teleost fish J. Exp. Biol., August 15, 2008; 211(16): 2584 - 2599. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. H. Evans Teleost fish osmoregulation: what have we learned since August Krogh, Homer Smith, and Ancel Keys Am J Physiol Regulatory Integrative Comp Physiol, August 1, 2008; 295(2): R704 - R713. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T.-Y. Lin, B.-K. Liao, J.-L. Horng, J.-J. Yan, C.-D. Hsiao, and P.-P. Hwang Carbonic anhydrase 2-like a and 15a are involved in acid-base regulation and Na+ uptake in zebrafish H+-ATPase-rich cells Am J Physiol Cell Physiol, May 1, 2008; 294(5): C1250 - C1260. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-J. Yan, M.-Y. Chou, T. Kaneko, and P.-P. Hwang Gene expression of Na+/H+ exchanger in zebrafish H+-ATPase-rich cells during acclimation to low-Na+ and acidic environments Am J Physiol Cell Physiol, December 1, 2007; 293(6): C1814 - C1823. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Nakada, K. Hoshijima, M. Esaki, S. Nagayoshi, K. Kawakami, and S. Hirose Localization of ammonia transporter Rhcg1 in mitochondrion-rich cells of yolk sac, gill, and kidney of zebrafish and its ionic strength-dependent expression Am J Physiol Regulatory Integrative Comp Physiol, October 1, 2007; 293(4): R1743 - R1753. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-L. Horng, L.-Y. Lin, C.-J. Huang, F. Katoh, T. Kaneko, and P.-P. Hwang Knockdown of V-ATPase subunit A (atp6v1a) impairs acid secretion and ion balance in zebrafish (Danio rerio) Am J Physiol Regulatory Integrative Comp Physiol, May 1, 2007; 292(5): R2068 - R2076. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Esaki, K. Hoshijima, S. Kobayashi, H. Fukuda, K. Kawakami, and S. Hirose Visualization in zebrafish larvae of Na+ uptake in mitochondria-rich cells whose differentiation is dependent on foxi3a Am J Physiol Regulatory Integrative Comp Physiol, January 1, 2007; 292(1): R470 - R480. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
