HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: 289/4/C1002    most recent 00175.2005v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (15) Citing Articles via Google Scholar Google Scholar Articles by Carrozzino, F. Articles by Montesano, R. Search for Related Content PubMed PubMed Citation Articles by Carrozzino, F. Articles by Montesano, R.

MEMBRANE TRANSPORTERS, ION CHANNELS, AND PUMPS

Inducible expression of Snail selectively increases paracellular ion permeability and differentially modulates tight junction proteins

¹Department of Cell Physiology and Metabolism, University of Geneva Medical Center, ²Department of Cell Biology, Faculty of Sciences, University of Geneva, and ³Laboratoire Central de Chimie Clinique, Hôpital Cantonal Universitaire, Geneva, Switzerland; ⁴Departamento de Bioquimica, Universidad Autónoma de Madrid (UAM), Instituto de Investigaciones Biomedicas "Alberto Sols" Consejo Superior de Investigaciones Científicas-UAM, Madrid, Spain; and ⁵Service de Néphrologie, Fondation pour Recherches Médicales, Geneva, Switzerland

Submitted 12 April 2005 ; accepted in final form 30 May 2005

Constitutive expression of the transcription factor Snail was previously shown to trigger complete epithelial-mesenchymal transition (EMT). The aim of this study was to determine whether inducible expression of Snail could modify epithelial properties without eliciting full mesenchymal conversion. For this purpose, we expressed mouse Snail (mSnail) cDNA in Madin-Darby canine kidney (MDCK) cells under the control of a doxycycline-repressible transactivator. Inducible expression of Snail did not result in overt EMT but induced a number of phenotypic alterations of MDCK cells, the most significant of which was the absence of fluid-filled blisterlike structures called "domes." To understand the mechanisms responsible for dome suppression, we assessed the effect of mSnail expression on epithelial barrier function. Although mSnail did not alter tight junction (TJ) organization and permeability to uncharged solutes, it markedly decreased transepithelial electrical resistance. In light of these findings, we evaluated the ability of MDCK cell monolayers to maintain ionic gradients and found that expression of mSnail selectively increases Na⁺ and Cl^– permeability. Analysis of the expression of claudins, transmembrane proteins that regulate TJ ionic permeability, showed that mSnail induces a moderate decrease in claudin-2 and a substantial decrease in claudin-4 and -7 expression. Together, these results suggest that induction of mSnail selectively increases the ionic permeability of TJs by differentially modulating the expression of specific claudins.

epithelium; Madin-Darby canine kidney cells; claudin; dome

This article has been cited by other articles:

				T. Horiuchi, K. Matsunaga, M. Banno, Y. Nakano, K. Nishimura, C. Hanzawa, K.-i. Miyamoto, S. Nomura, and Y. Ohta HPMCs INDUCE GREATER INTERCELLULAR DELOCALIZATION OF TIGHT JUNCTION-ASSOCIATED PROTEINS DUE TO A HIGHER SUSCEPTIBILITY TO H2O2 COMPARED WITH HUVECs Perit. Dial. Int., March 1, 2009; 29(2): 217 - 226. [Abstract] [Full Text] [PDF]

				A. Ferrari, A. Veligodskiy, U. Berge, M. S. Lucas, and R. Kroschewski ROCK-mediated contractility, tight junctions and channels contribute to the conversion of a preapical patch into apical surface during isochoric lumen initiation J. Cell Sci., November 1, 2008; 121(21): 3649 - 3663. [Abstract] [Full Text] [PDF]

				M. G. Laukoetter, P. Nava, W. Y. Lee, E. A. Severson, C. T. Capaldo, B. A. Babbin, I. R. Williams, M. Koval, E. Peatman, J. A. Campbell, et al. JAM-A regulates permeability and inflammation in the intestine in vivo J. Exp. Med., December 24, 2007; 204(13): 3067 - 3076. [Abstract] [Full Text] [PDF]

				R. E. Herman, E. G. Makienko, M. G. Prieve, M. Fuller, M. E. Houston Jr, and P. H. Johnson Phage Display Screening of Epithelial Cell Monolayers Treated with EGTA: Identification of Peptide FDFWITP that Modulates Tight Junction Activity J Biomol Screen, December 1, 2007; 12(8): 1092 - 1101. [Abstract] [PDF]

				V. Pollack, R. Sarkozi, Z. Banki, E. Feifel, S. Wehn, G. Gstraunthaler, H. Stoiber, G. Mayer, R. Montesano, F. Strutz, et al. Oncostatin M-induced effects on EMT in human proximal tubular cells: differential role of ERK signaling Am J Physiol Renal Physiol, November 1, 2007; 293(5): F1714 - F1726. [Abstract] [Full Text] [PDF]

				A. Zhang, M.-H. Wang, Z. Dong, and T. Yang Prostaglandin E2 is a potent inhibitor of epithelial-to-mesenchymal transition: interaction with hepatocyte growth factor Am J Physiol Renal Physiol, December 1, 2006; 291(6): F1323 - F1331. [Abstract] [Full Text] [PDF]