| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
REPORT
Departments of 1Internal Medicine and 4Molecular and Cellular Physiology, University of Cincinnati, Cincinnati, Ohio; Departments of 2Cellular and Molecular Physiology and 5Internal Medicine, Yale University School of Medicine, New Haven, Connecticut; 3Department of Gastroenterology, Hepatology, and Endocrinology, Hanover Medical School, Hanover, Germany; and 6Veterans Affairs Medical Center, Cincinnati, Ohio
Submitted 14 October 2004 ; accepted in final form 23 November 2004
ABSTRACT
SLC26A6 (PAT1, CFEX) is an anion exchanger that is expressed on the apical membrane of the kidney proximal tubule and the small intestine. Modes of transport mediated by SLC26A6 include Cl–/formate exchange, Cl–/HCO3– exchange, and Cl–/oxalate exchange. To study its role in kidney and intestinal physiology, gene targeting was used to prepare mice lacking Slc26a6. Homozygous mutant Slc26a6–/– mice appeared healthy and exhibited a normal blood pressure, kidney function, and plasma electrolyte profile. In proximal tubules microperfused with a low-HCO3–/high-Cl– solution, the baseline rate of fluid absorption (Jv), an index of NaCl transport under these conditions, was the same in wild-type and null mice. However, the stimulation of Jv by oxalate observed in wild-type mice was completely abolished in Slc26a6-null mice (P < 0.05). Formate stimulation of Jv was partially reduced in null mice, but the difference from the response in wild-type mice did not reach statistical significance. Apical membrane Cl–/base exchange activity, assayed with the pH-sensitive dye BCPCF in microperfused proximal tubules, was decreased by 58% in Slc26a6–/– animals (P < 0.001 vs. wild types). In the duodenum, the baseline rate of HCO3– secretion measured in mucosal tissue mounted in Ussing chambers was decreased by 
30% (P < 0.03), whereas the forskolin-stimulated component of HCO3– secretion was the same in wild-type and Slc26a6–/– mice. We conclude that Slc26a6 mediates oxalate-stimulated NaCl absorption, contributes to apical membrane Cl–/base exchange in the kidney proximal tubule, and also plays an important role in HCO3– secretion in the duodenum.
chloride absorption; bicarbonate secretion; proximal tubule; duodenum; apical anion exchange
This article has been cited by other articles:
|
|
 |
|
  D. Heitzmann and R. Warth Physiology and Pathophysiology of Potassium Channels in Gastrointestinal Epithelia Physiol Rev, July 1, 2008; 88(3): 1119 - 1182. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Mizumori, Y. Choi, P. H. Guth, E. Engel, J. D. Kaunitz, and Y. Akiba CFTR inhibition augments NHE3 activity during luminal high CO2 exposure in rat duodenal mucosa Am J Physiol Gastrointest Liver Physiol, June 1, 2008; 294(6): G1318 - G1327. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. R. Dorwart, N. Shcheynikov, D. Yang, and S. Muallem The Solute Carrier 26 Family of Proteins in Epithelial Ion Transport Physiology, April 1, 2008; 23(2): 104 - 114. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. E. Barrett New ways of thinking about (and teaching about) intestinal epithelial function Advan Physiol Educ, March 1, 2008; 32(1): 25 - 34. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Soleimani The role of SLC26A6-mediated chloride/oxalate exchange in causing susceptibility to nephrolithiasis J. Physiol., March 1, 2008; 586(5): 1205 - 1206. [Full Text] [PDF]
|
|
|
|
 |
|
 A. Toure, P. Lhuillier, J. A. Gossen, C. W. Kuil, D. Lhote, B. Jegou, D. Escalier, and G. Gacon The Testis Anion Transporter 1 (Slc26a8) is required for sperm terminal differentiation and male fertility in the mouse Hum. Mol. Genet., August 1, 2007; 16(15): 1783 - 1793. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Donowitz and X. Li Regulatory Binding Partners and Complexes of NHE3 Physiol Rev, July 1, 2007; 87(3): 825 - 872. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Zhang, N. Ameen, J. E. Melvin, and S. Vidyasagar Acute inflammation alters bicarbonate transport in mouse ileum J. Physiol., June 15, 2007; 581(3): 1221 - 1233. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. E. Simpson, C. W. Schweinfest, G. E. Shull, L. R. Gawenis, N. M. Walker, K. T. Boyle, M. Soleimani, and L. L. Clarke PAT-1 (Slc26a6) is the predominant apical membrane Cl-/HCO3- exchanger in the upper villous epithelium of the murine duodenum Am J Physiol Gastrointest Liver Physiol, April 1, 2007; 292(4): G1079 - G1088. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. A. Hassan, S. Mentone, L. P. Karniski, V. M. Rajendran, and P. S. Aronson Regulation of anion exchanger Slc26a6 by protein kinase C Am J Physiol Cell Physiol, April 1, 2007; 292(4): C1485 - C1492. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Ishiguro, W. Namkung, A. Yamamoto, Z. Wang, R. T. Worrell, J. Xu, M. G. Lee, and M. Soleimani Effect of Slc26a6 deletion on apical Cl-/HCO3- exchanger activity and cAMP-stimulated bicarbonate secretion in pancreatic duct Am J Physiol Gastrointest Liver Physiol, January 1, 2007; 292(1): G447 - G455. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Lamprecht and U. Seidler The emerging role of PDZ adapter proteins for regulation of intestinal ion transport Am J Physiol Gastrointest Liver Physiol, November 1, 2006; 291(5): G766 - G777. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. O'Reilly, E. Marshall, T. MacGillivray, M. Mittal, W. Xue, C. J. Kenyon, and R. W. Brown Dietary Electrolyte-Driven Responses in the Renal WNK Kinase Pathway In Vivo J. Am. Soc. Nephrol., September 1, 2006; 17(9): 2402 - 2413. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Petrovic, H. Amlal, X. Sun, F. Karet, S. Barone, and M. Soleimani Vasopressin induces expression of the Cl-/HCO3- exchanger SLC26A7 in kidney medullary collecting ducts of Brattleboro rats Am J Physiol Renal Physiol, May 1, 2006; 290(5): F1194 - F1201. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Shcheynikov, Y. Wang, M. Park, S. B.H. Ko, M. Dorwart, S. Naruse, P. J. Thomas, and S. Muallem Coupling Modes and Stoichiometry of Cl-/HCO3- Exchange by slc26a3 and slc26a6 J. Gen. Physiol., April 24, 2006; 127(5): 511 - 524. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. W. Freel, M. Hatch, M. Green, and M. Soleimani Ileal oxalate absorption and urinary oxalate excretion are enhanced in Slc26a6 null mice Am J Physiol Gastrointest Liver Physiol, April 1, 2006; 290(4): G719 - G728. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Xu, R. T. Worrell, H. C. Li, S. L. Barone, S. Petrovic, H. Amlal, and M. Soleimani Chloride/Bicarbonate Exchanger SLC26A7 Is Localized in Endosomes in Medullary Collecting Duct Cells and Is Targeted to the Basolateral Membrane in Hypertonicity and Potassium Depletion J. Am. Soc. Nephrol., April 1, 2006; 17(4): 956 - 967. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. L. Dudas, S. Mentone, C. F. Greineder, D. Biemesderfer, and P. S. Aronson Immunolocalization of anion transporter Slc26a7 in mouse kidney Am J Physiol Renal Physiol, April 1, 2006; 290(4): F937 - F945. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Sherman, M. N. Chernova, J. S. Clark, L. Jiang, S. L. Alper, and K. Nehrke The abts and sulp families of anion transporters from Caenorhabditis elegans Am J Physiol Cell Physiol, August 1, 2005; 289(2): C341 - C351. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
