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MEMBRANE TRANSPORTERS, ION CHANNELS, AND PUMPS

Calmodulin interactions with IQ peptides from voltage-dependent calcium channels

¹Department of Molecular Physiology and Biophysics, Baylor College of Medicine, Houston, Texas; and ²Department of Molecular and Cellular Biochemistry, Ohio State University, Columbus, Ohio

Submitted 16 April 2004 ; accepted in final form 6 October 2004

Calmodulin (CaM) functions as a Ca²⁺ sensor for inactivation and, in some cases, facilitation of a variety of voltage-dependent Ca²⁺ channels. A crucial determinant for CaM binding to these channels is the IQ motif in the COOH-terminal tail of the channel-forming subunit. The binding of CaM to IQ peptides from Lc-, P/Q-, and R-type, but not N-type, voltage-dependent Ca²⁺ channels increases the Ca²⁺ affinity of both lobes of CaM, producing similar N- and C-lobe Ca²⁺ affinities. Ca²⁺ associates with and dissociates from the N-lobe much more rapidly than the C-lobe when CaM is bound to the IQ peptides. Compared with the other IQ peptides, CaM-bound Lc-IQ has the highest Ca²⁺ affinity and the most rapid rates of Ca²⁺ association at both lobes, which is likely to make Ca²⁺ binding to CaM, bound to this channel, less sensitive than other channels to intracellular Ca²⁺ buffers. These kinetic differences in Ca²⁺ binding to the lobes of CaM when bound to the different IQ motifs may explain both the ability of CaM to perform multiple functions in these channels and the differences in CaM regulation of the different voltage-dependent Ca²⁺ channels.

Ca²⁺-dependent inactivation; Ca²⁺-dependent facilitation; apocalmodulin

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