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Am J Physiol Cell Physiol 288: C416-C428, 2005. First published September 29, 2004; doi:10.1152/ajpcell.00037.2004
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MEMBRANE TRANSPORTERS, ION CHANNELS, AND PUMPS

Expression of monocarboxylate transporters in rat ocular tissues

Glyn Chidlow,1 John P. M. Wood,1 Mark Graham,2 and Neville N. Osborne1

1Nuffield Laboratory of Ophthalmology, University of Oxford, Oxford; and 2AstraZeneca R&D Charnwood, Loughborough, United Kingdom

Submitted 20 January 2004 ; accepted in final form 22 September 2004

The aim of the present study was to determine the distribution of monocarboxylate transporter (MCT) subtypes 1-4 in the various structures of the rat eye by using a combination of conventional and real-time RT-PCR, immunoblotting, and immunohistochemistry. Retinal samples expressed mRNAs encoding all four MCTs. MCT1 immunoreactivity was observed in photoreceptor inner segments, Müller cells, retinal capillaries, and the two plexiform layers. MCT2 labeling was concentrated in the inner and outer plexiform layers. MCT4 immunolabeling was present only in the inner retina, particularly in putative Müller cells, and the plexiform layers. No MCT3 labeling could be observed. The retinal pigment epithelium (RPE)/choroid expressed high levels of MCT1 and MCT3 mRNAs but lower levels of MCT2 and MCT4 mRNAs. MCT1 was localized to the apical and MCT3 to the basal membrane of the RPE, whereas MCT2 staining was faint. Although MCT1-MCT4 mRNAs were all detectable in iris and ciliary body samples, only MCT1 and MCT2 proteins were expressed. These were present in the iris epithelium and the nonpigmented epithelium of the ciliary processes. MCT4 was localized to the smooth muscle lining of large vessels in the iris-ciliary body and choroid. In the cornea, MCT1 and MCT2 mRNAs and proteins were detectable in the epithelium and endothelium, whereas evidence was found for the presence of MCT4 and, to a lesser extent, MCT1 in the lens epithelium. The unique distribution of MCT subtypes in the eye is indicative of the pivotal role that these transporters play in the maintenance of ocular function.

retina; eye; immunohistochemistry; polymerase chain reaction


Address for reprint requests and other correspondence: G. Chidlow, Nuffield Laboratory of Ophthalmology, Univ. of Oxford, Walton St., Oxford OX2 6AW, UK (E-mail: glyn.chidlow{at}eye.ox.ac.uk)






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