HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: 287/5/C1426    most recent 00549.2002v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (6) Citing Articles via Google Scholar Google Scholar Articles by Nobles, M. Articles by Sardini, A. Search for Related Content PubMed PubMed Citation Articles by Nobles, M. Articles by Sardini, A.

MEMBRANE TRANSPORTERS, ION CHANNELS, AND PUMPS

Extracellular acidification elicits a chloride current that shares characteristics with I_Cl(swell)

Medical Research Council, Clinical Sciences Centre, Faculty of Medicine, Imperial College, Hammersmith Hospital Campus, London W12 0NN, United Kingdom

Submitted 25 November 2002 ; accepted in final form 15 July 2004

A Cl^– current activated by extracellular acidification, I_Cl(pHac), has been characterized in various mammalian cell types. Many of the properties of I_Cl(pHac) are similar to those of the cell swelling-activated Cl^– current I_Cl(swell): ion selectivity (I^– > Br^– > Cl^– > F^–), pharmacology [I_Cl(pHac) is inhibited by 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid (DIDS), 1,9-dideoxyforskolin (DDFSK), diphenylamine-2-carboxylic acid (DPC), and niflumic acid], lack of dependence on intra- or extracellular Ca²⁺, and presence in all cell types tested. I_Cl(pHac) differs from I_Cl(swell) in three aspects: 1) its rate of activation and inactivation is very much more rapid, currents reaching a maximum in seconds rather than minutes; 2) it exhibits a slow voltage-dependent activation in contrast to the fast voltage-dependent activation and time- and voltage-dependent inactivation observed for I_Cl(swell); and 3) it shows a more pronounced outward rectification. Despite these differences, study of the transition between the two currents strongly suggests that I_Cl(swell) and I_Cl(pHac) are related and that extracellular acidification reflects a novel stimulus for activating I_Cl(swell) that, additionally, alters the biophysical properties of the channel.

cell swelling-activated chloride current; patch clamp; pH

This article has been cited by other articles:

				A. P. D. Volk, C. K. Heise, J. L. Hougen, C. M. Artman, K. A. Volk, D. Wessels, D. R. Soll, W. M. Nauseef, F. S. Lamb, and J. G. Moreland ClC-3 and IClswell are Required for Normal Neutrophil Chemotaxis and Shape Change J. Biol. Chem., December 5, 2008; 283(49): 34315 - 34326. [Abstract] [Full Text] [PDF]

				S. B. Mazzone and A. E. McGovern Na+-K+-2Cl- cotransporters and Cl- channels regulate citric acid cough in guinea pigs J Appl Physiol, August 1, 2006; 101(2): 635 - 643. [Abstract] [Full Text] [PDF]

				S. Yamamoto and T. Ehara Acidic extracellular pH-activated outwardly rectifying chloride current in mammalian cardiac myocytes Am J Physiol Heart Circ Physiol, May 1, 2006; 290(5): H1905 - H1914. [Abstract] [Full Text] [PDF]